Evidence map›Paper›PMID 41105045›Full record

ArticleMicrobiologyOpen2025

In Vitro Antibacterial Activity of Raw Honey From Apis mellifera, Awi Zone, Amhara, Ethiopia.

Bulti Kumera, Bekele Gebreamanule, Belsti Atnkut, Mestawot Merid, Tadele Tilahun, Alemu Tsega, Amare Fassil, Yitayih Dessie, Banchalem Kassie

Abstract read
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In one paragraph

Article in MicrobiologyOpen, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bulti KumeraDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.ORCID 0000-0002-1706-5113
Bekele GebreamanuleDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.
Belsti AtnkutDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.ORCID 0000-0002-8441-6833
Mestawot MeridDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.
Tadele TilahunDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.
Alemu TsegaDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.ORCID 0009-0006-5013-4869
Amare FassilDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.ORCID 0000-0001-9359-7093
Yitayih DessieDepartment of Biology, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.ORCID 0009-0008-8801-0479
Banchalem KassieDepartment of Chemistry, College of Natural and Computational Science, Injibara University, Injibara, Ethiopia.

Funding

The authors received no specific funding for this work.
6 · The paper itself

Abstract

Honey has been used for the treatment of various illnesses since time immemorial. Honey is employed as a remedy for the treatment of burns, microbial infections, cardiovascular disorders, and gastrointestinal problems. The objectives of this study were to evaluate physicochemical characteristics and in vitro antibacterial properties of honey produced by Apis mellifera against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Klebsiella pneumoniae. Honey samples were collected from three districts of the Awi zone from April to June 2023. The pH and moisture of collected honey were determined following the standard procedure. An antimicrobial susceptibility test of honey samples was performed using an agar well diffusion assay. The moisture contents and pH of collected honey samples from the three study sites were 13 ± 0.07-20 ± 0.09 and 3.76-4.01, respectively. There was no significant (p > 0.05) difference in pH between honey samples collected from the three sites. Similarly, there was no significant statistical association among the moisture content of honey samples (p > 0.05). Pure 100% honey samples showed the greatest inhibition zone against E. coli (19.20 mm) and the lowest against P. aeruginosa (2.4 mm). Honey concentration of 25% showed the lowest inhibition zone against all tested bacterial pathogens. Antibacterial effects of honey solutions decreased upon serial dilution from 100% to 25%. Our finding suggests that raw honey can be used as therapeutic agents against bacterial disease.

Indexed as

Anti-Bacterial AgentsBacteriaHoneyAnimalsBeesEscherichia coliEthiopiaHydrogen-Ion ConcentrationKlebsiella pneumoniaeMicrobial Sensitivity TestsPseudomonas aeruginosaAnti-Bacterial Agentsagar well diffusion assayantibacterial activityhoneypathogen

Identifiers

PMID41105045
PMCPMC12532491

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.