ArticleeLife2025
scRNA+TCR-seq reveals the proportion and characteristics of dual TCR Treg cells in mouse lymphoid and non-lymphoid tissues.
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Proportion, Function, and Generation of Dual Receptor Lymphocytes.Journal of immunology research · 2026Review
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Authors and funding
6 authors.
Funding
Abstract
The rearrangement of TCR germline V(D)J genes during T cell development, including allelic exclusion and tolerance selection, ensures the clonal selection theory, which states that 'a lymphocyte expresses only one type of antigen receptor'. This forms the basis for T cell-specific responses. However, the existence of 'dual TCR T cells' has consistently been supported by specific experimental evidence. Detailed reports on the origin, proportion, tissue distribution, and CDR3 characteristics of 'dual TCR Treg cells' are currently lacking. In this study, we utilized scRNA+TCR-seq technology to achieve in-depth analysis of single and dual TCR T pairings, along with their mRNA expressions, from over 5000 T cells in each sample. Through comparative studies with shared databases, we provided a detailed analysis of the proportions and characteristics of dual TCR Tregs in mouse lymphoid and non-lymphoid tissues (such as inguinal lymph node, mesenteric lymph node, blood, and skin). Our findings revealed a high proportion of dual TCR Tregs across various mouse tissues, with their TCR pairing patterns, V(D)J usage, and mRNA expression showing both homogeneity and certain differences compared to single TCR Tregs, as well as heterogeneity across different tissue sites. This research provides new insights and technical approaches for studying the origins, characteristics, effects, and mechanisms of Treg cells in different tissue locations.
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