ArticleAnalytical chemistry2025
Effective Coupling of Light Emitting Diode to a Commercial Capillary Electrophoresis Laser-Induced Fluorescence Instrument for High-Sensitivity Analysis of Fluorescently Labeled Glycans.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Light-emitting diodes offer a low-cost, power-efficient, and compact solution for fluorescence excitation in analytical instrumentation. This study discusses the coupling of a near-ultraviolet light-emitting diode (340 nm) to a commercial capillary electrophoresis instrument and presents two feasible strategies: a simple, robust, and low-cost option with moderate efficiency and a more complex but significantly more efficient design for applications demanding maximum sensitivity. The coupling efficiency was assessed using capillary zone electrophoresis of maltooligosaccharides labeled with the UV-excitable fluorophore, 6-[4-(4-methylpiperazin-1-yl)phenyl]pyridine-3-carbohydrazide. Compared to a commonly used indirect light guide coupling approach, the new direct coupling design, incorporating a single ball lens, provided a 10.7-fold increase in the fluorescence signal. The design incorporating two plano-convex lenses increased the fluorescence signal by a factor of 31.2 and achieved limits of detection between 99 and 105 nmol/L for the analyzed labeled maltooligosaccharides. This optimized configuration enabled the successful
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