ArticleTurkish journal of obstetrics and gynecology2025
Comparison of survival and apoptosis parameters of ovarian tissue follicles in two vitrification methods of needle-immersion and cryo-support in cancer patients.
Article in Turkish journal of obstetrics and gynecology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: Ovarian tissue (OT) cryopreservation is a useful technique for preserving fertility potential in women with cancer. Vitrification is a relatively new method. Several devices were discussed. Among these methods, cryo-support and needle-immersion vitrification (NIV) stand out as particularly popular. The aim of this study is the comparison of these devices in terms of follicle quality and DNA status. Materials and Methods: The OT from 20 cancer patients was transferred with Dulbecco's Phosphate-Buffered Saline supplemented with 5% serum and maintained at 4 °C for 1 hour. After preparation of OT vitrified by two freezing solutions with different concentrations of cryoprotectant, small fragments of OT (~5×1×1 mm) were attached to an insulin needle, and in another group, the tissue fragments (~5×5×1 mm) were loaded onto the Ova Cryo Device Type M, also called cryo-support, and placed in liquid nitrogen. After warming, small tissue pieces were prepared to assess the quality of primordial, primary, and secondary follicles using eosin-hematoxylin staining. An immune-histochemical investigation, including anti-p53 and anti-Caspase 3, was conducted to examine the apoptosis pathway. Results: The data showed a larger number of high-quality follicles in the cryo-support group (p<0.05). Also, the level of apoptosis (p53) molecules is higher in the NIV method (p<0.05). However, the levels of caspase 3 were not significantly different between the NIV approach and the cryo-support vitrification group. Conclusion: A recent study revealed that the cryo-support device is more effective in increasing high-quality follicles and reducing p53, which is associated with early stages of apoptosis. This device may improve clinical outcomes and may be recommended for cryopreservation programs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.