ArticleJournal of proteome research2025
Profiling the Cellular Surfaceome by Furan-Based Protein Biotinylation.
Article in Journal of proteome research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- 2-Alkyl Furans Undergo Radiolytic Oxidative Protein Cross-Linking.Journal of the American Chemical Society · 2026Article
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Authors and funding
4 authors.
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Abstract
The cellular surfaceome is crucial for cellular homeostasis and communication, with surface proteins acting as receptors, transporters, and enzymes. However, comprehensive analysis of the surfaceome is challenging due to the hydrophobic nature and low abundance of membrane proteins, leading to their underrepresentation in mass spectrometry-based proteomics data. This study exploits a novel method for profiling the cellular surfaceome using furan chemistry. We synthesized six furan-biotin compounds (FB1-FB6) and showed that FB1 reacts with lysine and cysteine. Upon furan activation by oxidation, FB1 exhibited the highest staining intensity and specificity for cell surface proteins. Pull-downs of biotinylated proteins further confirmed the efficiency of FB1 in enriching cell surface proteins, and FB1 was also found to reduce nonspecific labeling of intracellular proteins compared to biotinylation methods using
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