ArticleTropical medicine and health2025
Systematic misclassification of sylvatic dengue virus 2 (DENV-2) infections as "undetermined serotype": implications in routine RT-qPCR surveillance.
Article in Tropical medicine and health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Recent findings by Suppiah et al. revealed DENV-2 positive samples misclassified as "Undetermined Serotype" when using commercial dengue molecular serotyping assays. This highlights the noninclusion and resulting misclassification of this viral strain as a dengue virus serotype using available molecular tools. Drawing parallels from the notification of the same assay failure against sylvatic DENV-2 strains in Senegal, West Africa, this correspondence advocates for updating the existing tools to account for this viral variant. Development of multiplex assays including both urban and sylvatic strains may enhance dengue virus surveillance and provide real time monitoring of the prevalence of sylvatic viral lineages among recorded dengue infections in surveillance data.
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