Comparative antioxidant activity of pomegranate leaf extract and derived metabolites in single and co-cultured cell lines.

Federica Foglietta, Giulia Querio, Cinzia Sanna, Roberto Canaparo, Arianna Marengo, Loredana Serpe, Patrizia Rubiolo

Abstract readComparative Study

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Federica Foglietta *Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy.

Giulia Querio *Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043, Orbassano, TO, Italy.

Cinzia SannaDepartment of Environmental and Life Sciences, University of Cagliari, Via Sant'Ignazio da Laconi 13, 09123, Cagliari, Italy.

Roberto CanaparoDepartment of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy.

Arianna MarengoDepartment of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy. arianna.marengo@unito.it.

Loredana Serpe *Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy.

Patrizia Rubiolo *Department of Drug Science and Technology, University of Turin, Via Pietro Giuria 9, 10125, Turin, Italy.

Funding

Università degli Studi di Torino "Ricerca Locale 2021"

6 · The paper itself

Abstract

The maintenance of cellular redox homeostasis with endogenous and exogenous antioxidants is crucial for regulating physiological functions. Plant extracts contain compounds that provide exogenous antioxidants, enhancing cellular protection against oxidative stress. This work aims to investigate the antioxidant potential of the ethanolic total extract (TE) and some derivatives (i.e., ellagic acid/EA, luteolin/LU) of the leaves of Punica granatum L. (pomegranate). Human dermal fibroblast (HDF) and human umbilical vein endothelial (HUVEC) cells were considered both as a single cell line culture and as a co-culture. TE and the single compounds were tested as primary or secondary antioxidants using hydrogen peroxide as a pro-oxidant, assessing cell viability, reactive oxygen species generation, and mRNA expression of antioxidant genes. Briefly, LU and EA showed a significant activity as primary antioxidants in H

Indexed as

AntioxidantsPlant ExtractsPlant LeavesPomegranateCell LineCell SurvivalCoculture TechniquesEllagic AcidFibroblastsGlutathione Peroxidase GPX1HumansHuman Umbilical Vein Endothelial CellsHydrogen PeroxideOxidative StressReactive Oxygen SpeciesAntioxidantsEllagic AcidGlutathione Peroxidase GPX1Hydrogen PeroxidePlant ExtractsReactive Oxygen SpeciesAntioxidantEllagic acidLuteolinOxidative stressPunica granatum L. leaf extract

Identifiers

PMID41102240

DOI10.1038/s41598-025-20130-6

PMCPMC12532804

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.