ArticlePloS one2025
Anti-cancer activity of 7-methoxyheptaphylline from Clausena harmandiana against PANC-1 pancreatic cancer cells and its sustainable extraction method.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Anticancer Effects ofInternational journal of molecular sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pancreatic cancer presents a significant therapeutic challenge characterized by poor survival rates, leading to development of innovative treatment strategies. This study evaluated the anti-cancer potential of 7-methoxyheptaphylline (7-MH), a carbazole alkaloid from Clausena harmandiana, against pancreatic cancer cells (PANC-1) and developed an environmentally sustainable extraction methodology using ultrasonic-assisted extraction (UAE). 7-MH demonstrated selective cytotoxicity against PANC-1 cells with preferential activity under nutrient deprivation conditions (PC50 = 4.54 μM) compared to nutrient-rich conditions (IC50 = 46.84 μM). This compound exhibited minimal toxicity toward normal MCE301 epithelial cells (IC50 = 83.4 μM). Live-cell imaging showed dose-dependent apoptotic morphology including membrane blebbing and cell shrinkage within 24 hours. At concentrations of 25 and 50 μM, the compound significantly inhibited wound closure and colony formation, suggesting antimetastatic properties. Mechanistic analysis exhibited that 7-MH suppressed the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway specially under nutrient deprived conditions. Western blot analysis showed 45% reduction in Akt expression, 43% decrease in mTOR phosphorylation, and complete inhibition of Akt phosphorylation at 20 µM concentration. For sustainable extraction of 7-MH, UAE using ethanol was optimized through response surface methodology (RSM) with central composite design (CCD). The optimal protocol (50 °C, 60 minutes, 0.40 g/10 mL plant/solvent ratio) achieved 1.26 ± 0.02% yield with only 3.55% deviation from predicted values. This developed extraction method provides an efficient and environmentally sustainable alternative to conventional halogenated solvent extraction methods. These findings offer valuable insights for advancing natural product-based cancer therapeutics and demonstrate the implementation of sustainable natural products extraction principles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.