Evidence map›Paper›PMID 41099938›Full record

ArticleActa parasitologica2025

In Vitro and In Silico Anticoccidial Activity of Opuntia ficus-indica Seed Oil and Hexane Extract: Comprehensive GC-MS Profiling and Mechanistic Insights.

Dalia Kellil, Rabah Arhab, Asma Kheddouma, Hamza Ahmed-Laloui, Abderrahmen Rahmani, Chawki Bensouici, Abdennour Azizi, Mohamed Abdesselem Dems, Ayomide Victor Atoki, Ramzi Bezghiche and 4 more

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Article in Acta parasitologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dalia KellilLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Rabah ArhabLaboratory of Natural Substances, Biomolecules and Biotechnological Applications, Larbi Ben M'Hidi University, Oum El Bouaghi, Algeria.
Asma KheddoumaLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Hamza Ahmed-LalouiAnimal Production Team, Biotechnology and Agriculture Division; Biotechnology Research Center, Ali Mendjli, Constantine, Algeria.
Abderrahmen RahmaniAnimal Production Team, Biotechnology and Agriculture Division; Biotechnology Research Center, Ali Mendjli, Constantine, Algeria.
Chawki BensouiciLaboratory of Biochemistry, Biotechnology and Health Division, Biotechnology Research Center, Ali Mendjeli, Constantine, Algeria.
Abdennour AziziVeterinary Science Department, Veterinary Sciences and Agricultural Sciences Institute, University of Batna 1, 05000, Batna, Algeria.
Mohamed Abdesselem DemsBiotechnology Research Center, Ali Mendjeli, Constantine, Algeria.
Ayomide Victor AtokiDepartment of Biochemistry, Kampala International University, Ishaka, Uganda. atokiav@kiu.ac.ug.
Ramzi BezghicheLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Houssam Eddine BentounsiLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Houria BouteraaLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Abdelwahab BenhocineLaboratory of Biotechnology, Water, Environment and Health, University of Abbes Laghrour, Khenchela, Algeria.
Mohammed MessaoudiLaboratoire de Recherche Sur Les Produits Bioactifs Et Valorisation de La Biomasse, Département de Chimie, ENS Kouba, Alger, Algeria. messaoudi2006@yahoo.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The anticoccidial potential of Opuntia ficus-indica seed oil (OFI-SO) and its hexane extract from seed press cake (OFI-HexPC) was evaluated against Eimeria spp. using integrated in vitro and in silico approaches. Sporulated Eimeria oocysts were exposed to increasing concentrations (2-64 mg/mL) of OFI-SO and OFI-HexPC in Hank's balanced salt solution. Oocyst viability was determined by microscopic counting, while membrane integrity was assessed by quantifying the release of 273 nm-absorbing substances. Toltrazuril (25 mg/mL) was used as a positive control. GC-MS profiling of both extracts revealed linoleic acid (54.2%) and oleic acid (25.7%) as the main bioactive fatty acids. GC-MS profiling revealed that linoleic acid (69.63%) was the major fatty acid in OFI-SO, while oleic acid (44.20%) predominated in OFI-HexPC. Both OFI-SO and OFI-HexPC significantly reduced oocyst counts and induced a dose-dependent increase in DNA release. In silico molecular docking was performed against three key Eimeria enzymes (EtDHODH, CDPK, and PKA). Identified fatty acids showed moderate binding affinities through hydrophobic interactions. Redocking validation (RMSD < 2 Å) confirmed the reliability of the docking protocol. ADMET predictions indicated favorable pharmacokinetics and safety profiles. These findings suggest that OFI-SO and OFI-HexPC act via a dual mechanism involving membrane disruption and enzymatic inhibition, supporting their potential as natural alternatives to synthetic anticoccidials in poultry production.

Indexed as

CoccidiostatsEimeriaOpuntiaPlant ExtractsPlant OilsAnimalsGas Chromatography-Mass SpectrometryHexanesMolecular Docking SimulationOocystsSeedsCoccidiostatsHexanesPlant ExtractsPlant OilsAnticoccidial activityEimeria SppGC–MSIn vitroMolecular dockingOpuntia ficus-indicaPhytogenic compounds

Identifiers

PMID41099938

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.