Evidence map›Paper›PMID 41099882›Full record

ArticleAntonie van Leeuwenhoek2025

Function and transcription of the lon gene in Xanthomonas campestris pv. campestris.

Hsiao-Ching Chang, Ju-Hsuan Yeh, Hsueh-Hsia Lo, Chao-Tsai Liao, Yi-Min Hsiao

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Article in Antonie van Leeuwenhoek, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Hsiao-Ching ChangDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
Ju-Hsuan YehDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
Hsueh-Hsia LoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
Chao-Tsai LiaoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.
Yi-Min HsiaoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 406, Taiwan. ymhsiao@ctust.edu.tw.

Funding

National Science and Technology Council NSTC113-2313-B-166-001
6 · The paper itself

Abstract

The Gram-negative bacterium Xanthomonas campestris pv. campestris is the etiological agent of black rot, a widespread and destructive disease affecting cruciferous plants. In this study, a lon mutant was obtained by EZ-Tn5 transposon mutagenesis of the X. campestris pv. campestris. The lon gene encodes an ATP-dependent protease implicated in protein quality control and stress adaptation across various bacterial species. Functional analysis revealed that lon disruption in X. campestris pv. campestris resulted in diminished extracellular protease activity, reduced virulence, and heightened sensitivity to puromycin and elevated temperatures. Complementation with the wild-type lon allele restored most phenotypes, except thermotolerance, which was only partially recovered. Interestingly, lon overexpression in wild-type cells compromised growth under heat stress, indicating that lon dosage is critical for thermal adaptation. Promoter activity assays indicated that lon expression is subject to catabolite repression and is induced by heat shock. Additionally, analysis of upstream regions of lon and multiple experimentally validated heat-inducible genes revealed a conserved motif similar to the σ

Indexed as

Bacterial ProteinsGene Expression Regulation, BacterialProtease LaTranscription, GeneticXanthomonas campestrisHeat-Shock ResponsePromoter Regions, GeneticVirulenceBacterial ProteinsProtease LaPathogenicityPromoterReporter assayStress toleranceXanthomonas

Identifiers

PMID41099882

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