Construction of yeast with extremely high 2,3-butanediol tolerance by introducing point and structural mutations and partial elucidation of the mechanism of 2,3-butanediol tolerance.

Kaito Nakamura, Ryosuke Yamada, Rumi Sakaguchi, Takuya Matsumoto, Hiroyasu Ogino

Abstract read

In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed.

Mutagenesis and Analysis for Enhancing (2Molecules (Basel, Switzerland) · 2026
Article

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kaito NakamuraDepartment of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.

Ryosuke YamadaDepartment of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan. ryamada@omu.ac.jp.ORCID http://orcid.org/0000-0003-3820-7501

Rumi SakaguchiDepartment of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.

Takuya MatsumotoDepartment of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.

Hiroyasu OginoDepartment of Chemical Engineering, Osaka Metropolitan University, 1-1 Gakuen-Cho, Naka-Ku, Sakai, Osaka, 599-8531, Japan.

Funding

GteX Program of Japan JPMJGX23B4

6 · The paper itself

Abstract

Microbial production of valuable chemicals is a promising and sustainable approach, offering high energy efficiency and minimal waste generation. Production of 2,3-butanediol (2,3-BDO) by the safe industrial yeast Saccharomyces cerevisiae holds potential as a sustainable bioprocess. However, the low tolerance of 2,3-BDO in yeast remains a major challenge. In this study, we aimed to improve 2,3-BDO tolerance in S. cerevisiae by introducing DNA point and structural mutations using techniques developed in previous studies, thereby advancing the sustainable industrial production of 2,3-BDO. Through point and structural mutagenesis, we successfully obtained the mutant strain YPH499/Co58, which exhibited a 122-fold higher OD

Indexed as

Butylene GlycolsPoint MutationSaccharomyces cerevisiaeGene Expression ProfilingMetabolic EngineeringMutagenesis2,3-butylene glycolButylene Glycols2,3-ButanediolMutagenesisSaccharomyces cerevisiaeStress toleranceTranscriptome analysisYeast

Identifiers

PMID41099839

DOI10.1007/s00253-025-13626-8

PMCPMC12532680

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.