Workflow for efficiently isolating microspore cultures of different rice genotypes by optimizing the callus induction medium.

Zhiwei Chen, Guimei Guo, Shuwei Zhang, Ting He, Shiji Feng, Chenghong Liu, Yu Wang, Longhua Zhou

Abstract read

In one paragraph

Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhiwei ChenShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Guimei GuoShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Shuwei ZhangShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Ting HeShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Shiji FengShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Chenghong LiuShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Yu WangShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Longhua ZhouShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As one of the most important staple foods in the world, rice plays a key role in global food security. Doubled haploid technology based on isolated microspore culture can shorten the time taken for rice breeding programs. However, this technology still faces many problems, such as genotypic dependency, low culture efficiency, and a shortage of skilled workers. In this study, 15 rice genotypes, comprising 12

Indexed as

callus inductionmicrospore cultureOryza sativa L.plant regenerationploidy identification

Identifiers

PMID41098908

DOI10.3389/fpls.2025.1662463

PMCPMC12518337

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.