Evidence map›Paper›PMID 41098530›Full record

ArticleFrontiers in microbiology2025

Comparison of 16S ribosomal RNA hypervariable regions in microbiome studies of anorexia nervosa.

Arunabh Sharma, Nadia Andrea Andreani, Lara Keller, Beate Herpertz-Dahlmann, Jochen Seitz, John F Baines, Astrid Dempfle

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Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Arunabh SharmaInstitute of Medical Informatics and Statistics, Kiel University, Kiel, Germany.
Nadia Andrea AndreaniMax Planck Institute for Evolutionary Biology, Plön, Germany.
Lara KellerDepartment of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital, RWTH Aachen University, Aachen, Germany.
Beate Herpertz-DahlmannDepartment of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital, RWTH Aachen University, Aachen, Germany.
Jochen SeitzDepartment of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, University Hospital, RWTH Aachen University, Aachen, Germany.
John F BainesMax Planck Institute for Evolutionary Biology, Plön, Germany.
Astrid DempfleInstitute of Medical Informatics and Statistics, Kiel University, Kiel, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Short read sequencing of the 16S ribosomal RNA (rRNA) gene targeting specific hypervariable regions is widely employed to study the human gut microbiota. In these studies, the selection of particular 16S rRNA hypervariable regions is a crucial step. However, the results of such studies exhibit significant variability depending on the targeted hypervariable region. Methods: In this study, we systematically evaluated the performance of hypervariable regions V1V2 and V3V4 in a longitudinal gut microbiome study of adolescent patients with anorexia nervosa (AN) and matched controls. Results: The dominant genera, such as Conclusion: This study underscores the importance of primer selection in microbiome studies of AN, as it can influence taxonomic resolution and diversity estimates along with downstream statistical analyses.

Indexed as

16S rRNAanorexia nervosaeating disordergut-brain axisgut microbiomelongitudinal studyV1V2V3V4

Identifiers

PMID41098530
PMCPMC12519842

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