Evidence map›Paper›PMID 41097002›Full record

ArticleInternational journal of molecular sciences2025

The Establishment of a Sheep Embryo Genomic Selection System.

Yubing Wang, Hao Qin, Ke Li, Jia Hao, Xingyuan Liu, Dayong Chen, Lei Cheng, Huijie He, Riga Wu, Yingjie Wu and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Yubing WangLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Hao QinLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ke LiLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Jia HaoLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Xingyuan LiuLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Dayong ChenKey Laboratory of Sheep Genetic Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs, Ulanqab 011800, China.
Lei ChengKey Laboratory of Sheep Genetic Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs, Ulanqab 011800, China.
Huijie HeKey Laboratory of Sheep Genetic Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs, Ulanqab 011800, China.
Riga WuKey Laboratory of Sheep Genetic Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs, Ulanqab 011800, China.
Yingjie WuLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.ORCID 0000-0003-2675-2625
Yinjuan WangLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Min GuoLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Qin LiLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Lei AnLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Jianhui TianLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Hongbing HanLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Guangyin XiLaboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture and Rural Affairs, Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.ORCID 0000-0001-5997-6540

Funding

Central guidance of local science and technology development funds No. 2024ZY0160Inner Mongolia Autonomous Region Science and Technology Innovation Major Demonstration Project No. 2025SZDSF0010Sci-Tech Innovation 2030 Agenda No. 2023ZD040750202, No. 2022ZD040130301
6 · The paper itself

Abstract

Embryo genomic selection (EGS) is a contemporary breeding strategy that combines genomic selection (GS) methodology with embryo biotechnology. By conducting genotyping and genomic prediction at the pre-implantation stage, embryos with superior breeding value can be identified for transfer, markedly increasing breeding efficiency while reducing the uncertainty and temporal expenditure associated with conventional GS. This study establishes a reliable embryo biopsy-based GS pipeline for sheep, incorporating optimized whole-genome amplification and microcell genotyping techniques. We developed a high-efficiency in vitro sheep embryo production platform compatible with embryo biopsy. Systematic comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping Based Amplification Cycles (MALBAC) whole-genome amplification systems yielded high-quality genotypes from biopsy samples of embryos containing as few as 10 cells. Imputation using 10× whole-genome sequencing data significantly increased both genotype call rates and accuracy. High concordance was observed between embryo and lamb genotypes, and genomic estimated breeding values (GEBVs) for key growth traits exhibited strong correlations (R

Indexed as

BreedingEmbryo, MammalianGenomeGenomicsSelection, GeneticAnimalsFemaleGenotypePolymorphism, Single NucleotideSheepWhole Genome Sequencinganimal breedingchip-based genotypingembryo biopsyembryo genomic selectionin vitro embryo productionsheepwhole-genome amplification

Identifiers

PMID41097002
PMCPMC12525284

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.