Evidence map›Paper›PMID 41096593›Full record

ArticleInternational journal of molecular sciences2025

Safety and Regenerative Properties of Immortalized Human Mesenchymal Stromal Cell Secretome.

Maxim Karagyaur, Alexandra Primak, Nataliya Basalova, Anna Monakova, Anastasia Tolstoluzhinskaya, Maria Kulebyakina, Elizaveta Chechekhina, Mariya Skryabina, Olga Grigorieva, Vadim Chechekhin and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Maxim KaragyaurMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0003-4289-3428
Alexandra PrimakMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0002-1386-5922
Nataliya BasalovaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0002-2597-8879
Anna MonakovaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0002-7123-6374
Anastasia TolstoluzhinskayaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Maria KulebyakinaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0001-5956-1265
Elizaveta ChechekhinaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0002-5377-2712
Mariya SkryabinaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Olga GrigorievaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Vadim ChechekhinMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0001-8489-9954
Tatiana YakovlevaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave., 4, 194064 St. Petersburg, Russia.ORCID 0000-0002-4772-5645
Victoria TurilovaInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave., 4, 194064 St. Petersburg, Russia.ORCID 0000-0002-6318-9395
Elena ShagimardanovaSkolkovo Institute of Science and Technology (Skoltech), Genomics and Bioimaging Core Facility, Skolkovo Innovation Center, Bolshoy Boulevard, 30, Bld. 1, 121205 Moscow, Russia.ORCID 0000-0003-2339-261X
Guzel GazizovaInstitute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia.
Maksim VigovskiyMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0003-2103-8158
Konstantin KulebyakinMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0001-6954-5787
Veronika SysoevaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0001-9885-9056
Uliana DyachkovaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Stalik DzhauariMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Kirill BozovMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Vladimir PopovMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Zhanna AkopyanMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.
Anastasia EfimenkoMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0002-0696-1369
Natalia KalininaMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.ORCID 0000-0003-3497-9619
Vsevolod TkachukMedical Research and Education Institute, Lomonosov Moscow State University, 27/1, Lomonosovsky Ave., 119192 Moscow, Russia.

Funding

Russian Science Foundation 19-75-30007
6 · The paper itself

Abstract

The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regeneration and therefore is a tempting remedy for "cell-free cellular therapy". However, the usage of primary MSC cultures as secretome producers for translation studies has obvious obstacles, including the rapid aging of MSC cultures, the need for a large number of verified donors, and donor-to-donor variability of secretome content. MSCs immortalization makes it possible to overcome those limitations and to obtain secretome-producing cultures with a prolonged lifetime. However, the efficacy and safety of such secretomes are critical issues that limit their usage as therapeutic agents. In this study, we tested in large detail how the immortalization of MSC cultures affects the content, biological activity and safety of their secretome. MSCs immortalization via the overexpression of human

Indexed as

Mesenchymal Stem CellsRegenerationSecretomeCells, CulturedHumansProteomeProteomicsTelomeraseProteomeTelomeraseTERT protein, humancell secretomefibroblast-to-myofibroblast differentiationimmortalized cell culturesLeydig cell culture potency assaymesenchymal stromal cellsregeneration

Identifiers

PMID41096593
PMCPMC12525087

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.