Multifunctional Electrospun Materials from Poly(Vinyl Alcohol)/Chitosan and Polylactide Incorporating Rosmarinic Acid and Lidocaine with Antioxidant and Antimicrobial Properties.

Novel multifunctional fibrous materials were prepared by simultaneous dual spinneret electrospinning of two separate solutions differing in composition. This technique allowed for the preparation of materials built of two types of fibers: fibers from poly(vinyl alcohol) (PVA), chitosan (Ch), and rosmarinic acid (RA), and poly(L-lactide) (PLA) fibers containing lidocaine hydrochloride (LHC). Confocal laser scanning microscopy (CLSM) analyses showed that both types of fibers are present on the surface and in the bulk of the new materials. The presence of all components and some interactions between them were proven by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. RA and LHC were in an amorphous state in the fibers, and their presence affected the temperature characteristics and the crystallinity, as detected by differential scanning calorimetry (DSC) and X-ray diffraction analyses (XRD). The presence of PVA/Ch/RA fibers enabled the hydrophilization of the surface of the multifunctional fibrous materials (the water contact angle value was 0°). The newly developed materials demonstrated adequate mechanical properties, making them suitable for use in wound dressing applications. The RA-containing fibrous mats possessed high radical-scavenging activity (ca. 93%), and the combining with LHC led to an enhancement of this effect (ca. 98.5%). RA-containing fibrous mats killed all the pathogenic bacteria