ArticleInfectious agents and cancer2025
FAM19A4 and miR124-2 methylation status in human papillomavirus-driven and human papillomavirus-negative oropharyngeal squamous cell carcinomas.
Article in Infectious agents and cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- HPV-Driven Cervical Carcinogenesis: Genetic and Epigenetic Mechanisms and Diagnostic Approaches.International journal of molecular sciences · 2026Review
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9 authors.
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Abstract
backgroundScarce data exist on host gene methylation in oropharyngeal squamous cell carcinomas (OPCs), which are caused by human papillomavirus (HPV) in approximately 40% of cases. We analyzed the methylation status of two host genes involved in cervical carcinogenesis, FAM19A4 and miR124-2, in formalin-fixed paraffin-embedded (FFPE) tissues of primary HPV-driven OPCs (i.e., HPV-positive and p16INK4A-positive), HPV-negative OPCs, head and neck (HN) squamous cell papillomas, and oral rinse-and-gargles (ORGs) from individuals without HN lesions.
methodsA multiplex real-time methylation-specific PCR on bisulfite-converted DNA was employed (PreCursor-M+, Fujirebio). Hypermethylation for each target was expressed as negative/positive based on the ΔΔCt, as well as by the ΔΔCt ratio (2
resultsA total of 70 HPV-driven OPCs (66 HPV16+, 94.3%), 71 HPV-negative OPCs, 12 HN papillomas, and 20 ORGs were evaluated. Six of the 153 FFPE-purified DNA samples (3.9%) yielded invalid results. Hypermethylation for at least one target gene was observed in 56 of 69 valid HPV-driven OPCs (81.2%) and 33 of 67 valid HPV-negative OPCs (49.2%; p < 0.0001). None of the papillomas or ORGs were hypermethylated. HPV-driven OPCs showed a significantly higher methylation level compared with HPV-negative OPCs for both FAM19A4 (median ΔΔCt ratio 11.95 vs. 5.49; p = 0.0001) and miR124-2 (median ΔΔCt ratio 15.65 vs. 8.27; p < 0.0001).
conclusionsOur findings indicate that FAM19A4/miR124-2 hypermethylation occurs exclusively in tumor samples and that methylation levels are significantly higher in HPV-driven OPCs than in HPV-negative OPCs.
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