Evidence map›Paper›PMID 41094508›Full record

ArticleVirology journal2025

Comparative infectivity of EBV subtypes in tree shrews (Tupaia belangeri).

Zhenqiu Luo, Nan Shi, Fangfang Chen, Zongjian Huang, Wei Xia, Anzhou Tang

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Zhenqiu Luo *Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Nan Shi *Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Fangfang ChenDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Zongjian HuangDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Wei XiaDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China. YFY004532@sr.gxmu.edu.cn.
Anzhou TangDepartment of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China. tanganzhou@gxmu.edu.cn.

Funding

Guangxi Medical University Youth Science Foundation GXMUYSF202425Guangxi Natural Science Fund No.2020JJ A140398Guangxi Science and Technology Program under Grant No.AD25069077Innovation Project of Guangxi Graduate Education No.YCBZ2024120 , No.YCSW2024267Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi 2023KY0087Natural Science Foundation of China U21A20371, No.82160217, No.82260519
6 · The paper itself

Abstract

backgroundThe Epstein-Barr Virus (EBV) has a high global prevalence and is strongly associated with the pathogenesis of diverse diseases. Types 1 (T1) and 2 (T2) represent the two major EBV subtypes. However, their infectivity and pathogenic mechanisms remain unclear. We aimed to explore the differences between T1 and T2 EBV infections using the tree shrew model.

methodsT1 and T2 EBVs were purified from the supernatant of EBV-producing cells lines B95-8 and P3HR-1, respectively cell line. Eighteen tree shrews (evenly divided by sex) were randomly assigned to three groups (n = 6). Each animal intravenously received 1 × 10⁸ copies of either T1 EBV, T2 EBV, or RPMI-1640 medium (vehicle control) via the femoral vein. Peripheral blood was collected at 3, 7, 14, 21, and 28 days post-inoculation (dpi) for quantitative EBV-DNA detection. peripheral blood mononuclear cells (PBMCs) were collected on 7 and 28 dpi for RNA-seq analysis, and comparisons were made with pre-infection samples to assess host transcriptional changes.

resultsEBV-DNA was detectable in the PBMCs of all six tree shrews by day 3 post-inoculation in the T1 EBV (B95-8) group, peaking at 1 × 10

conclusionsT2 EBV demonstrated significantly lower infectivity than T1 EBV in the tree shrew model. T1 infection elicits inflammatory responses, whereas T2 infection induces modest transcriptional alterations.

Indexed as

Epstein-Barr Virus InfectionsHerpesvirus 4, HumanTupaiaTupaiidaeAnimalsCell LineDisease Models, AnimalDNA, ViralFemaleLeukocytes, MononuclearMaleDNA, ViralEpstein–Barr virus (EBV)Host transcriptional responseSubtype-Specific infectivityTree shrew modelViral pathogenesis

Identifiers

PMID41094508
PMCPMC12522292

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.