Evidence map›Paper›PMID 41094164›Full record

ArticlePediatric research2026

Combined transamniotic delivery of surfactant proteins B and C mRNA enhances preterm fetal surfactant production in a rodent model.

Kamila Moskowitzova, Emily M Scire, Tanya T Dang, Beatriz S Bechara, Eva Zacharakis, Ayaka Aihara, David Zurakowski, Dario O Fauza

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Article in Pediatric research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Kamila MoskowitzovaDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Emily M ScireDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Tanya T DangDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Beatriz S BecharaDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Eva ZacharakisDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Ayaka AiharaDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
David ZurakowskiDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
Dario O FauzaDepartment of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA. dario.fauza@childrens.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPrematurity remains the leading cause of neonatal mortality and morbidity, largely due to pulmonary surfactant deficiency. We compared isolated and combined transamniotic delivery of surfactant proteins B (SPB) and C (SPC) mRNA.

methodsFetuses (n = 415) from thirty-two time-dated dams underwent intra-amniotic injections on gestational day 17 (E17, term= E21-22) of lipopolyplex-encapsulated mRNA consisting of either human SPB (hSPB) mRNA (n = 151), human SPC (hSPC) mRNA (n = 102), a combination of hSPB+hSPC mRNA (n = 113), or lipopolyplex without mRNA (n = 49). Fetal lungs were screened for hSPB and hSPC by ELISA daily until term. Phosphatidylcholine was measured in the amniotic fluid.

resultsControlled by mRNA-free injections, the hSPB protein was detected in the SPB mRNA group only at E18 and E19, whereas in the SPB + SPC mRNA group it was detected at every preterm timepoint (E18-E20), either separately or combined (p < 0.001 for all). The hSPC protein was detected in the SPC mRNA group only at E18 (p = 0.015). Amniotic fluid phosphatidylcholine was significantly increased in the SPC mRNA and SPB + SPC mRNA groups compared to controls (p < 0.001).

conclusionsCombined transamniotic delivery of exogenous mRNA encoding for SPB and SPC enhances surfactant production prior to term when compared with isolated mRNA administrations in a healthy rat model. IMPACT: Transamniotic mRNA delivery may become a novel strategy for surfactant replacement therapy. Combined transamniotic mRNA delivery for surfactant proteins B and C enhances preterm surfactant production. Transamniotic mRNA delivery can be achieved in a short time frame, for example compatible with select clinical settings of preterm labor.

Indexed as

Pulmonary Surfactant-Associated Protein BPulmonary Surfactant-Associated Protein CPulmonary SurfactantsRNA, MessengerAmnionAmniotic FluidAnimalsFemaleFetusHumansLungPhosphatidylcholinesPregnancyRatsRats, Sprague-DawleyPhosphatidylcholinesPulmonary Surfactant-Associated Protein BPulmonary Surfactant-Associated Protein CPulmonary SurfactantsRNA, Messenger

Identifiers

PMID41094164

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.