ArticleJournal of Crohn's & colitis2025
Spatial transcriptomics reveals unique inflammatory signatures across all anatomic locations in postoperative Crohn's disease.
Article in Journal of Crohn's & colitis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Leveraging Single-Cell and Spatial Transcriptomics in Gut Nutrition and Mucosal Immunology.The Journal of nutrition · 2026Article
- Spatial Immunology in Translation: Linking Immune Organisation to Therapeutic Outcome.Medical sciences (Basel, Switzerland) · 2026Review
- Shared inflammatory architecture and therapeutic tensions between psoriasis and Crohn's disease.Frontiers in immunology · 2026Review
- Microenvironment-Responsive Nanomaterials for Colorectal Anastomotic Healing: A Signal-Threshold-Response Framework for Stage-Adaptive Repair.International journal of nanomedicine · 2026Review
- Spatial multi-omics-guided drug development in inflammatory bowel disease: from mucosal immune niches to precision therapy.Frontiers in immunology · 2026Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsMost patients with Crohn's disease (CD) who have undergone ileocolonic resection experience recurrent inflammation within 1 year after surgery. We examined the molecular basis underlying gastrointestinal inflammation in postoperative CD across 3 common anatomic locations of recurrence.
methodsTo characterize spatial transcriptomic signatures, this study utilized biopsies from the colon, neo-terminal ileum, and anastomosis of patients with postoperative CD in the PREDICT-OR study. Sample analyses were performed with 10X Genomics Visium CytAssist system V2.0, and data analyses with R.
resultsHistologically inflamed biopsies from all locations shared transcriptional signatures across 3 cellular niches (myeloid, B, T cells) and a specialized epithelial cell type expressing inflammation-associated genes. Differentially expressed genes overexpressed inflammatory pathway activity across the 3 locations, whereas hypoxic pathways were less apparent. In addition to genes for known treatment targets, epidermal growth factor receptor and mitogen-activated protein kinase pathways were upregulated. Cellular niches shaped inflammatory microenvironments through endoplasmic reticulum stress and extracellular matrix remodeling signaling.
conclusionsApplication of spatial transcriptomics revealed a common disease signature for postoperative CD across the colon, neo-terminal ileum, and anastomosis. Inflamed biopsies from all locations demonstrated similar immune cell and inflammatory gene expression patterns as opposed to hypoxic pathways, and unique inflammatory pathways were revealed.
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