ArticleMolecular biology of the cell2025
Localization and function of septins are susceptible to epitope tagging.
Article in Molecular biology of the cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Protocol for assessing protein expression levels and localization during fission yeast cytokinesis.STAR protocols · 2026Article
- Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans.The Journal of cell biology · 2026Article
- Molecular regulation of PGC-1α: from protein-protein interactions and post-translational modifications to pharmacological modulation.Journal of molecular medicine (Berlin, Germany) · 2026Review
- Localizations of the septin Spn4 tagged with GFP and mEGFP in fission yeast.microPublication biology · 2025Article
Corrections and comments
- Update of
Authors and funding
6 authors.
Funding
Abstract
Septins are hetero-oligomeric cytoskeletal proteins that assemble into filaments and scaffolds on the plasma membrane to aid cytokinesis, morphogenesis, and other cellular processes. Epitope tagging is widely used to study septin localization and function. However, it is technically challenging to test the functionality of tagged septins outside fungi. Fission yeast provides an ideal genetic system to test functionalities and localizations of tagged septins. mEGFP/mYFP-tagged septins Spn1 and Spn4 localize to the division site as double rings during cytokinesis, but tdTomato tagged septins also localize to puncta or short linear structures across the plasma membrane. It was proposed that these additional septin structures serve as diffusion barriers and are important for the localizations and functions of several proteins, including the NDR-kinase Sid2 and active Cdc42 GTPase. By analyzing cell morphology, cytokinesis defects, and genetic interactions between tagged septins and three mutations, we find that septins are less functional with tdTomato or 3HA than with other tags. Additionally, Sid2 appearance at the division site is after septins and delayed in septin deletions, contrary to previous reports. Our data re-emphasize the need for rigorous functional tests of tagged septins and for caution in interpreting function and localization data when using epitope-tagged septins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.