Evidence map›Paper›PMID 41089454›Full record

ArticleFrontiers in microbiology2025

Revealing Amur tiger family pedigrees based on age identification using fecal microbiome and kinship analysis.

Xiaoyun Hu, Zhijian He, Chang Liu, Yifei Zhang, Dejun Mu, Valentin Yu Guskov, Kai Wang, Yong Yao, Dan Jin, Junguang Lu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiaoyun Hu *College of Life Science, Jilin Agricultural University, Changchun, China.
Zhijian He *Feline Research Center, National Forestry and Grassland Administration, College of Wildlife and Protected Areas, Northeast Forestry University, Harbin, Heilongjiang, China.
Chang Liu *Changchun Zoological and Botanical Park, Changchun, Jilin, China.
Yifei Zhang *Department of Economics and Management, City University of Wuhan, Wuhan, Hubei, China.
Dejun MuChangchun Zoological and Botanical Park, Changchun, Jilin, China.
Valentin Yu GuskovFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences (FSC EATB FEB RAS), Vladivostok, Russia.
Kai WangLiaoning Qianshan Tourism Group Co., Anshan, Liaoning, China.
Yong YaoChongqing Zoo Management Office, Chongqing, China.
Dan JinChangchun Zoological and Botanical Park, Changchun, Jilin, China.
Junguang LuChangchun Zoological and Botanical Park, Changchun, Jilin, China.
Yao NingCollege of Life Science, Jilin Agricultural University, Changchun, China.
Guangshun JiangFeline Research Center, National Forestry and Grassland Administration, College of Wildlife and Protected Areas, Northeast Forestry University, Harbin, Heilongjiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The construction of a species' family pedigree is crucial for understanding population structure, assessing genetic diversity, and conserving the genetic resources of endangered species. However, developing non-invasive and reliable methods for age identification in wild individuals remains a significant challenge in family pedigree establishments. Methods: In this study, we employed 16S rRNA sequencing and metagenomic analysis to examine 30 fecal samples collected from captive Amur tigers across three distinct age groups, aiming to identify the age-specific biomarker, which could subsequently facilitate age determination of wild individuals and support the construction of species pedigree. Results: Our results demonstrate that, through 16S rRNA high-throughput sequencing, 16 potential microbial age biomarkers were identified in fecal samples from captive Amur tigers, and the ages of 17 captive individuals were distinguished. Notably, Discussion: This study established a comprehensive scientific framework for pedigree reconstruction based on age determination in Amur tigers and developed a scalable, non-invasive methodology offering opportunities for population structure and promoting the precision of conservation for wild tigers.

Indexed as

ageAmur tigermicrobiomicsmicrosatellitepedigree

Identifiers

PMID41089454
PMCPMC12515850

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.