ArticleJournal of translational medicine2025
Integrated profiling of semen microbiota and metabolome reveals dysregulation in primary idiopathic male infertility.
Article in Journal of translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- A narrative review of the gut microbiota-testis axis: mechanisms and implications for male reproductive health.Translational andrology and urology · 2026Review
- Interplay Between Sperm Function and Seminal Plasma Biochemical Profile in a Group of Normozoospermic Subjects.Biomolecules · 2026Article
- Unfolding the Semen Microbiota: Implications for Male Infertility.Biomedicines · 2026Review
- Epidemiology, Etiopathogenesis, Diagnosis, and Treatment of Male Infertility-Current Trends and Future Directions: A Narrative Review.Medicina (Kaunas, Lithuania) · 2026Review
- Prostatitis-Related Male Infertility: From Inflammation and Dysbiosis to Sperm DNA Damage.Diagnostics (Basel, Switzerland) · 2026Review
- Animal models of gut microbiota-male reproductive interactions: methodological assessment, translational bottlenecks, and optimization strategies.Frontiers in endocrinology · 2026Review
- The therapeutic potential ofFrontiers in microbiology · 2026Review
- Interpretable machine learning uncovers core seminal microbial signatures in idiopathic oligoasthenospermia.Frontiers in cellular and infection microbiology · 2026Article
Corrections and comments
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Authors and funding
19 authors.
Funding
Abstract
backgroundPrimary idiopathic male infertility remains a significant challenge due to its complex etiology and the lack of effective diagnostic tools. This study characterized seminal microbiota and metabolome alterations in this condition and their association with sperm quality, aiming to identify potential biomarkers.
methodsWe conducted an integrated microbiota-metabolome profiling study, enrolling 26 men with primary idiopathic infertility and 14 fertile controls. Semen samples were evaluated according to WHO guidelines. Microbiota composition was assessed by 5R 16S rRNA sequencing, and metabolites were profiled using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Statistical analyses included diversity assessment, differential abundance testing, correlation analysis, and ROC curve evaluation for potential biomarkers.
resultsInfertile men exhibited significantly lower seminal microbiota α-diversity, with 45 differentially abundant taxa identified. Metabolomics identified 147 differentially expressed metabolites (DEMs). Four DEMs (γ-Glu-Tyr, Indalone, Lys-Glu, γ-Glu-Phe) showed exceptional diagnostic potential (AUC > 0.97). Providencia rettgeri, Pediococcus pentosaceus, and Streptococcus pneumoniae abundance correlated positively with sperm quality, whereas Proteus penneri correlated negatively. Metabolites Arg-Arg, Fumarate, and Lpc 18:2 positively correlated with sperm motility, whereas Lys-Glu and Indalone showed negative correlations.
conclusionThis integrated analysis reveals distinct dysbiosis of the seminal microbiota and metabolic disruptions in idiopathic male infertility, offering novel insights into its mechanisms and biomarker discovery.
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