ArticleVeterinary research2025
Experimental validation of RNA interference technologies for improved control of barber's pole worm.
Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Host insulin hijacking by a nematode receptor mediates developmental plasticity and sex ratio shifts.Nature communications · 2026Article
- In vitro transcriptome and proteome of Haemonchus contortus larvae exposed to host blood components.Scientific data · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
Over the past decade, small RNA technologies have been proposed for improved control of parasitic worms. Although achievements have been made in the identification of target candidates and in the improvement of reverse genetic tools, few of those have been tested in domestic animals. In this work, crucial genes (i.e., daf-9/cyp-22a1, bli-5 and HCON_00083600) involved in the developmental transition (i.e., activation, moulting and haem utilisation) from the infective L3 stage to the parasitic L4 stage of Haemonchus contortus (the barber's pole worm commonly found in small ruminants) in vitro were identified and verified using RNA interference (RNAi) technologies during the adaptation to parasitism of this parasite in vivo. Silencing each of the daf-9/cyp-22a1, bli-5 and HCON_00083600 genes in the infective larvae of H. contortus resulted in compromised larval development and viability in vitro, and silencing of either the daf-9/cyp-22a1, bli-5 or HCON_00083600 gene led to a marked reduction in the faecal egg count and worm burden in sheep. In conclusion, the results demonstrate that genes involved in larval activation, moulting and haem utilisation of H. contortus are target candidates, and the application of RNAi technologies for better control of these and related parasitic nematodes is promising, preferably with an improved RNAi tool for efficient and long-lasting effects in host animals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.