Evidence map›Paper›PMID 41087395›Full record

ArticleScientific reports2025

Methylene blue as a new signal tracer for nucleic acid-based lateral flow assay.

Jurjaan Onayza Noim, Dhruvi Kakadiya, Stephanie Dang, Nabil Royez, Shruti Ahuja, Krishna Prasad Aryal, Siddharth Tallur, Dylan Ravindran Pillai, Richa Pandey

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jurjaan Onayza NoimDepartment of Biomedical Engineering, University of Calgary, 2500 University Dr NW, T2N 1N4, Calgary, AB, Canada.
Dhruvi KakadiyaDepartment of Biomedical Engineering, University of Calgary, 2500 University Dr NW, T2N 1N4, Calgary, AB, Canada.
Stephanie DangDepartment of Biomedical Engineering, University of Calgary, 2500 University Dr NW, T2N 1N4, Calgary, AB, Canada.
Nabil RoyezDepartment of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
Shruti AhujaCentre for Research in Nanotechnology & Science (CRNTS), Indian Institute of Technology (IIT) Bombay, Mumbai, India.
Krishna Prasad AryalDepartment of Biomedical Engineering, University of Calgary, 2500 University Dr NW, T2N 1N4, Calgary, AB, Canada.
Siddharth TallurDepartment of Electrical Engineering, Indian Institute of Technology (IIT) Bombay, Mumbai, India.
Dylan Ravindran PillaiDepartment of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
Richa PandeyDepartment of Biomedical Engineering, University of Calgary, 2500 University Dr NW, T2N 1N4, Calgary, AB, Canada. richa.pandey@ucalgary.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the development of MebiQue-LFA, a nucleic acid-integrated lateral flow assay that utilizes methylene blue (MB) as a new colorimetric signal tracer for detecting nucleic acids. MebiQue-LFA advances LFA technology by enabling visual detection via blue dye and enhancing sensitivity through integrated electrochemical transduction on the same platform. The electrochemical readout provides a sensitive and quantifiable alternative when visual detection is limited at low analyte concentrations. To demonstrate dual-mode detection, the assay was designed to target a model single-stranded DNA sequence, achieving a detection limit (DL) of 0.62 fM and 43 fM (~ 100 copies/µL) in buffer and contrived diluted (50%) blood respectively, with a dynamic range spanning 1 pM to 1 µM. The platform exhibited high specificity, discriminating single-base mismatches and non-complementary sequences. Furthermore, MebiQue-LFA demonstrated robust performance in complex biological matrices, including plasma and serum, achieving a DL of 16 pM (~ 10

Indexed as

Biosensing TechniquesMethylene BlueNucleic AcidsColorimetryDNA, Single-StrandedElectrochemical TechniquesHumansLimit of DetectionDNA, Single-StrandedMethylene BlueNucleic AcidsElectrochemical biosensorLateral flow assayMethylene blueNucleic acid detectionQuantitative DNA detection

Identifiers

PMID41087395
PMCPMC12521506

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.