Evidence map›Paper›PMID 41086845›Full record

ArticleProceedings. Biological sciences2025

Ancient DNA and biomarkers from artefacts: insights into technology and cultural practices in Neolithic Europe.

Anna E White, Tabea J Koch, Theis Zetner Trolle Jensen, Jonas Niemann, Mikkel Winther Pedersen, Maja Birk Søtofte, Didier Binder, Cédric Lepère, Christian Harb, Renata Huber and 8 more

Abstract readHistorical Article
In one paragraph

Article in Proceedings. Biological sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Anna E White *Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.ORCID 0000-0001-6922-7885
Tabea J Koch *BioArCh, Department of Archaeology, University of York, York YO10 5DD, UK.ORCID 0000-0001-9907-2554
Theis Zetner Trolle JensenGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.ORCID 0000-0002-7166-7975
Jonas NiemannGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.ORCID 0000-0002-5153-100X
Mikkel Winther PedersenGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.ORCID 0000-0002-7291-8887
Maja Birk SøtofteGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.
Didier BinderCNRS, CEPAM, Université Côte d'Azur, Nice 06300, France.ORCID 0000-0001-8232-5367
Cédric LepèreCNRS, CEPAM, Université Côte d'Azur, Nice 06300, France.
Christian HarbDienststelle Kultur, Archäologie, Luzern, Switzerland.
Renata HuberAmt für Denkmalpflege und Archäologie, Zug, Switzerland.ORCID 0000-0002-4226-5918
Léonard KramerService Archéologique de l'Etat de Fribourg, Fribourg, Switzerland.
Michel MauvillyService Archéologique de l'Etat de Fribourg, Fribourg, Switzerland.
Renate EbersbachLandesamt für Denkmalpflege Baden-Württemberg, Hemmenhofen 78343, Germany.ORCID 0000-0002-8746-3141
Joachim WahlLandesamt für Denkmalpflege Baden-Württemberg, Konstanz 78467, Germany.
Aimée LittleCentre for Artefacts and Materials Analysis, Department of Archaeology, University of York, York YO10 5DD, UK.
Nathan WalesBioArCh, Department of Archaeology, University of York, York YO10 5DD, UK.ORCID 0000-0003-0359-8450
Martine RegertCNRS, CEPAM, Université Côte d'Azur, Nice 06300, France.ORCID 0000-0002-9209-6964
Hannes SchroederGlobe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 1353, Denmark.ORCID 0000-0002-6743-0270

Funding

CarlsbergfondetHORIZON EUROPE European Research CouncilHORIZON EUROPE Marie Sklodowska-Curie Actions
6 · The paper itself

Abstract

Birch bark tar was widely used throughout prehistoric Europe for hafting stone tools as well as various other purposes. While previous research has mainly focused on the identification and production of birch bark tar, its diverse uses remain to be fully explored. In this study, we combined ancient DNA with organic residue analysis to analyse 30 birch tar artefacts from nine Neolithic sites in and around the Alps. We identified birch tar as the main component, with some samples also containing conifer resin or tar, possibly added to modify its properties. Degradation markers indicate that tar used for ceramic repair was heated repeatedly, probably during cooking. Additionally, the presence of human and oral microbial DNA in some of the samples suggests the tar was chewed, in some cases by multiple individuals. The human DNA also enables us to determine the sex of those who chewed the tar, offering insights into gendered practices in the past, while plant and animal DNA shed light on past diets and the possible use of additives. This study underscores the value of integrating organic residue and ancient DNA analysis of archaeological artefacts to deepen our understanding of past cultural practices.

Indexed as

DNA, AncientAnimalsArchaeologyBiomarkersEuropeHistory, AncientHumansPlant BarkBiomarkersDNA, AncientarchaeologyGC-MSlipid analysismetagenomicstechnology

Identifiers

PMID41086845
PMCPMC12520791

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.