Evidence map›Paper›PMID 41086812›Full record

ArticleCell reports. Medicine2025

Isotype conversion of Staphylococcal-specific IgG into IgM broadens the reactivity to other bacterial pathogens.

Remy M Muts, Astrid Hendriks, Josefien W Hommes, Max L B Grönloh, Douwe J Dijkstra, Carla J C de Haas, Piet C Aerts, Eduard H T M Ebberink, Albert J R Heck, Zhen Wang and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Remy M MutsDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Astrid HendriksDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Josefien W HommesDepartment of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
Max L B GrönlohDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Douwe J DijkstraDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Carla J C de HaasDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Piet C AertsDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Eduard H T M EbberinkBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands.
Albert J R HeckBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands; Netherlands Proteomic Center, 3584 CH Utrecht, the Netherlands.
Zhen WangLeiden Institute of Chemistry, Leiden University, 2333 CC Leiden, the Netherlands.
Haoru ZhuangLeiden Institute of Chemistry, Leiden University, 2333 CC Leiden, the Netherlands.
Jeroen D C CodéeLeiden Institute of Chemistry, Leiden University, 2333 CC Leiden, the Netherlands.
Bas G J SurewaardDepartment of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.
Dani A C HeesterbeekDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Nina M van SorgeDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands; Netherlands Reference Laboratory for Bacterial Meningitis, Amsterdam UMC, Amsterdam, the Netherlands.
Suzan H M RooijakkersDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands. Electronic address: s.h.m.rooijakkers@umcutrecht.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Therapeutic antibodies are actively explored as alternative to treat or prevent bacterial infections. However, the narrow antigen specificity of IgG in combination with broad diversity in bacterial surface structures currently hampers the development of therapeutic antibodies against bacteria. Here we reveal that isotype conversion of three highly specific anti-staphylococcal antibodies from IgG into IgM does not only affect Fc effector functions but also modifies the interaction of Fab domains with bacterial surface antigens. These converted IgMs gain cross-reactivity for a broad range of bacterial species, including Gram-negatives such as Escherichia coli and Neisseria meningitidis and even protect against invasive infection with Streptococcus pyogenes in vivo. Mechanistic studies show that enhanced cross-specificity by IgM is conferred by changed ligand specificity and multivalent binding to high-density antigens. Altogether, these findings provide important insights for the development of antibody therapy for bacterial infections.

Indexed as

Antibodies, BacterialImmunoglobulin GImmunoglobulin MStaphylococcusAnimalsAntibody SpecificityAntigens, BacterialCross ReactionsEscherichia coliHumansMiceNeisseria meningitidisStaphylococcal InfectionsStreptococcus pyogenesAntibodies, BacterialAntigens, BacterialImmunoglobulin GImmunoglobulin Mantibody therapiesbacteriaIgMinfectionspecies-specificity

Identifiers

PMID41086812
PMCPMC12629822

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.