Evidence map›Paper›PMID 41085640›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2026

Isolation and characterization of a lytic phage and its efficacy against multi-drug resistant strains of pseudomonas aeruginosa.

Mohammad Sarmast, Hadi Peeri-Dogaheh, Chiman Karami, Farzad Khademi

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Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Mohammad SarmastDepartment of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
Hadi Peeri-DogahehDepartment of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. h.peeridogaheh@arums.ac.ir.
Chiman KaramiDepartment of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. c.karami@arums.ac.ir.
Farzad KhademiDepartment of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPhages are potent alternatives to antibiotics due to their lytic activity and specificity. We isolated and characterized a specific bacteriophage effective against resistant strains of P. aeruginosa and their biofilms.

methodsSamples were collected from hospital sewage. The morphological and life-cycle characteristics, pH and thermal stability, and host range of the isolated phage were assessed. Additionally, the sensitivity of 42 P. aeruginosa strains and their biofilms was evaluated.

resultsMorphological analysis showed that vB-pae-PA01 resembled Podophages. Sensitivity assays revealed that vB-pae-PA01 lysed 57% of the strains. vB-pae-PA01 exhibited strong thermal stability. However, highly acidic and basic conditions reduced its lytic activity. The one-step growth curve revealed a latent period of 10 min and a burst size of 105 PFU per cell.

conclusionWe isolated the lytic bacteriophage vB-pae-PA01, demonstrating potent lytic activity against P. aeruginosa strains. These properties suggest that vB-pae-PA01 could be a potential candidate to use against antibiotic-resistant P. aeruginosa.

Indexed as

Drug Resistance, Multiple, BacterialPseudomonas aeruginosaPseudomonas PhagesAnti-Bacterial AgentsBiofilmsHost SpecificityHumansHydrogen-Ion ConcentrationMicrobial Sensitivity TestsPseudomonas InfectionsSewageAnti-Bacterial AgentsSewageAntibiotic-resistanceBacteriophageBiofilmPhage-therapyPseudomonas aeruginosa

Identifiers

PMID41085640

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.