ArticleBioanalysis2025
Performance properties of filter-paper used in blood spot collection devices for quantitation of phenylalanine.
Article in Bioanalysis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 authors.
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Abstract
aimsAccurate and precise measurement of dried blood spot (DBS) phenylalanine (Phe) is vital for managing phenylketonuria (PKU). Standard DBS collection devices use grade-226 filter-paper, while the CapitainerB quantitative device utilizes grade-222 filter-paper. Although grade-226 filter-paper performance is well characterized, data on grade-222 filter-paper are sparse. This study aimed to investigate the analytical properties of grade-222 and grade-226 filter-papers. MATERIALS AND
methodsWe compared grade-222 and grade-226 filter-papers for Phe measurement accuracy and imprecision in DBS generated using both filter-papers. Scanning electron microscopy (SEM) and slit lamp imaging were used to assess the physical properties of the filter-papers.
resultsUsing an aqueous calibrator as reference, grade-222 exhibited a mean bias of -1.1%, the mean bias for grade-226 was -7.3%. Intra-assay imprecision was 2.3% for grade-222, versus 4.2% for grade-226. SEM revealed that fibers in grade-226 filter-paper are bonded by an amorphous material, which is absent in grade-222 filter-paper. Total error analysis indicated grade-222 filter-paper reduced uncertainty of Phe measurement compared to grade-226 filter-paper.
conclusionsGrade-222 filter-paper was proven to have superior analytical performance for Phe quantification, providing improved differentiation between safe and harmful Phe concentrations and offering more reliable PKU monitoring compared to traditional grade-226 filter-paper.
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