Evidence map›Paper›PMID 41084967›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Spatially Resolved Mapping of Voltage-Gated Proton Channel Activity Reveals Delayed Proton Transport in Local Microenvironments.

Jiahua Zhuang, Yang Xu, Yuxian Lu, Shiyang Lyu, Jie Tan, Jiandong Feng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jiahua ZhuangLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.
Yang XuLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.
Yuxian LuHanghzou Institute of Advanced Studies, Zhejiang Normal University, Hangzhou, 311231, China.
Shiyang LyuLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.
Jie TanLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.ORCID https://orcid.org/0000-0002-0909-2904
Jiandong FengLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.ORCID https://orcid.org/0000-0002-2143-3986

Funding

National Key R&D Program of China 2020YFA0211200National Natural Science Foundation of China 62205298the Fundamental Research Funds for the Zhejiang Provincial Universities 226-2025-00087Zhejiang Provincial Natural Science Foundation of China LQ23F050008
6 · The paper itself

Abstract

Voltage-gated proton channel Hv1 is a proton-selective channel that lacks the pore domain typically found in other ion channels, and it is thought to participate in signal transduction by inducing transient, localized changes in proton concentration. However, the contribution of Hv1 channel distribution and activity to the overall pH homeostatic process is still not well understood. Here a single-molecule photobleaching-based quantitative imaging method is developed with an Hv1-fused ratiometric pHluorin probe to study the distribution and dynamics of the proton channel in living bacteria. It is shown that the Hv1 channel does not close sharply when voltage is removed and can directly cause local fluctuations of intracellular pH gradient. Especially, the proton transport through Hv1 is significantly delayed in hypertonic environments, offering novel perspectives at cellular level for neuropathological research. This spatially localized pH microenvironment created by the Hv1 channel may play a crucial role in signal transduction in neuron cells.

Indexed as

Ion ChannelsHydrogen-Ion ConcentrationIon TransportProtonsSignal TransductionIon ChannelsProtonscellular electrophysiologyneurodegenerative diseasesproton channelssingle‐molecule imaging

Identifiers

PMID41084967
PMCPMC12786307

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.