ArticleIndian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion2025
Establishment of Flow Cytometry-Based Assay for the Quantitative Measurement of Erythrocyte Agglutination in Clinical Laboratory.
Article in Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Detection of RBC-bound antibodies using flow cytometry and their correlation with HLA polymorphism.Frontiers in immunology · 2026Article
- A new way of the Coombs test using flow cytometry-based assay to assess erythrocytes-bound IgG antibodies in the human and rabbit model.International journal of immunopathology and pharmacologyArticle
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4 authors.
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Abstract
This study explores a novel sensitive and accurate method for the quantitative determination of red blood cells (RBCs) agglutination in clinical laboratory. Natural antibodies (Anti-B) were selected and diluted as 1:2, 1:4,1:8 and 1:16 to react with healthy blood group RBC-B, using normal saline as a control group. All the sample tubes were incubated at 37 °C for 30 min. On other hand, tube method was also used and the degree of agglutination was graded. The strongest agglutination was observed in the 1:1 dilution, but as the serum was serially diluted from 1:2 to 1:16, the agglutination strength decreased. In contrast, no agglutination was found in the control group. Furthermore, we plotted the logarithm of serum dilution on X-axis coordinate while the index of agglutination (IAG) on Y-axis coordinate, which showed that there is a good linear relationship existed between IAG and serum (Anti-B) Concentration (r = 0.979 and
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