ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Dual-Pedicle Tissue-Engineered Trachea Promotes Biomimetic Cartilaginous Framework, Vascularization, and Epithelial Lining for Long-Segment Tracheal Reconstruction.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids.Research (Washington, D.C.) · 2026Article
- Towards a bioengineered airway: advances in tracheal tissue engineering and biofabrication.Frontiers in cell and developmental biology · 2026Review
- Dual-Pedicle Tissue-Engineered Trachea Promotes Biomimetic Cartilaginous Framework, Vascularization, and Epithelial Lining for Long-Segment Tracheal Reconstruction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Long-segment tracheal reconstruction remains a formidable clinical challenge due to failures of conventional tissue-engineered trachea (TET) grafts, stemming from inadequate vascularization, compromised cartilaginous framework, and epithelial deficiency. Transcriptomic profiling of conventional TETs revealed enriched hypoxia and inflammatory pathways, underscoring the need for robust vascular and epithelial strategies. Herein, a dual-pedicle biomimetic TET is developed to provide a stable cartilaginous framework for airway support, immediate blood supply for tissue perfusion, and functional epithelial lining for barrier protection. Autologous rabbit auricular skin is sutured into tubes for epithelial lining, while chondrocytes in photo-crosslinkable decellularized Wharton's jelly matrix hydrogels formed C-shaped constructs assembled into cartilage tubes. Both are pre-vascularized separately in cervical muscle for 4 weeks, then assembled with preserved unilateral pedicles, yielding integrated TETs with dual-pedicle vascular networks and enhanced biomechanical properties. Orthotopic tracheal transplantation of this construct into rabbits demonstrated sustained patency, minimal stenosis, and improved 28 day survival (60% vs 40% for single-pedicle controls). Immunofluorescence confirmed reduced bacterial infiltration, apoptosis, and inflammation (TLR4, MPO), with restored epithelial barriers (Claudin-1, β-defensin 1) and airway differentiation (cytokeratin 8 upregulation). Transcriptomics validated regenerative pathways, including oxidative phosphorylation and tight junctions. This dual-pedicle approach preempts pathological cascades, offering a promising paradigm for clinical tracheal regeneration.
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Registered trials
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