Evidence map›Paper›PMID 41082157›Full record

ArticleStem cell reviews and reports2026

Exosome Purification Strategy Influences the Regenerative Efficacy of Dental Pulp Stem Cell-Derived Exosomes in Bone Regeneration.

Borae Lee, Chia-Yi Hung, Yu-Ting Chien, Chi-Che Hsieh, Wei-Hao Cheng, Ya-Hui Chan, Pin-Chuang Lai, Sheng-Wei Feng

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Article in Stem cell reviews and reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Borae Lee *School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Chia-Yi Hung *School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Yu-Ting ChienSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Chi-Che HsiehSchool of Dentistry, College of Oral Medicine, Taipei Medical University, No.250, Wuxing St, Taipei, 11031, Taiwan.
Wei-Hao ChengSchool of Dentistry, College of Oral Medicine, Taipei Medical University, No.250, Wuxing St, Taipei, 11031, Taiwan.
Ya-Hui ChanSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Pin-Chuang LaiDepartment of Periodontics, School of Dentistry, University of Missouri- Kansas City, Kansas City, MO, USA.
Sheng-Wei FengSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan. shengwei@tmu.edu.tw.

Funding

National Science and Technology Council NSTC 112-2314-B-038-040-MY3Taipei Medical University-Shuang Ho Hospital, Ministry of Health and Welfare 112TMU-SHH-28
6 · The paper itself

Abstract

backgroundMesenchymal stem cell (MSC)-based tissue engineering is largely mediated by extracellular vesicles (EVs), particularly exosomes (Exos). The biological activity of Exos is strongly influenced by the isolation method, yet no standardized approach exists.

methodsDental pulp stem cell (DPSC)-derived Exos were isolated using ultracentrifugation (UC) or size exclusion chromatography (SEC). UC-Exos and SEC-Exos were characterized for morphology, size, yield, protein content, and proteomic profiles. Their effects on periodontal ligament stem cells (PDLSCs) were assessed, including proliferation, migration, and osteogenic differentiation. In vivo bone regeneration was evaluated in rat calvarial defects using Exos combined with bone-grafting material.

resultsSEC-Exos yielded exosomes with higher purity, greater particle counts, and lower protein contamination than UC-Exos. Proteomic profiling showed enrichment of osteogenic factors, including ENPP2 and afamin, in SEC-Exos. Functionally, UC-Exos promoted PDLSC proliferation and migration, while SEC-Exos more effectively enhanced osteogenic differentiation. Both Exos improved PDLSC functions dose-dependently and, when combined with bone grafts, consistently promoted calvarial bone regeneration. Notably, osteocalcin expression was higher in SEC-Exos-treated defects.

conclusionsThe isolation method critically determines the biological performance of DPSC-derived Exos. UC-Exos may be preferable for stimulating proliferation and migration, whereas SEC-Exos show advantages in osteogenic differentiation. Both approaches facilitated bone regeneration in vivo, suggesting that exosome isolation strategies should be tailored to experimental requirements and therapeutic objectives to optimize translational outcomes. SIGNIFICANCE STATEMENT: The isolation method critically determines the biological performance of DPSC-derived exosomes. SEC-Exos, enriched in osteogenic factors such as ENPP2 and afamin, preferentially enhanced osteogenic differentiation, whereas UC-Exos promoted proliferation and migration. Both approaches supported consistent bone regeneration in vivo, underscoring that the optimal isolation strategy should be tailored to specific experimental and therapeutic objectives to advance translational applications.

Indexed as

Bone RegenerationDental PulpExosomesStem CellsAnimalsCell DifferentiationCell MovementCell ProliferationCells, CulturedHumansMaleMesenchymal Stem CellsOsteogenesisPeriodontal LigamentRatsRats, Sprague-DawleyBone regenerationDental pulp stem cellsExosomesPeriodontal ligament stem cellsSize exclusion chromatographyUltracentrifugation

Identifiers

PMID41082157

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.