Evidence map›Paper›PMID 41082114›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Lysosome Enrichment Using Superparamagnetic Iron Oxide Nanoparticles (SPIONs).

Pathma Muthukottiappan, Sara Bonini, Dominic Winter

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Pathma MuthukottiappanDepartment of Metabolism, Senescence and Autophagy, Research Center One Health Ruhr, University Alliance Ruhr, University Hospital Essen, Medical Faculty, University of Duisburg-Essen, Essen, Germany.
Sara BoniniDepartment of Metabolism, Senescence and Autophagy, Research Center One Health Ruhr, University Alliance Ruhr, University Hospital Essen, Medical Faculty, University of Duisburg-Essen, Essen, Germany.
Dominic WinterDepartment of Metabolism, Senescence and Autophagy, Research Center One Health Ruhr, University Alliance Ruhr, University Hospital Essen, Medical Faculty, University of Duisburg-Essen, Essen, Germany. dominic.winter@uk-essen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lysosomes are dynamic and low-abundant organelles, and their enrichment is essential to facilitate a comprehensive biochemical characterization. Several methods are available to achieve this goal, such as density gradient centrifugation, immunoprecipitation, and superparamagnetic iron oxide nanoparticles (SPIONs). Compared to the other approaches, SPIONs present several advantages, as they enable the fast enrichment of intact lysosomes at high yields and purity without the need for the expression of tagged proteins. This protocol describes an optimized workflow for the enrichment of lysosomes from mammalian cells using SPIONs. We describe cell culture and treatment conditions tailored toward commonly utilized cell lines, approaches for cell rupture and lysosome enrichment, and downstream analyses by enzymatic assays and western blotting to assess the success of lysosome enrichment.

Indexed as

Cell FractionationLysosomesMagnetic Iron Oxide NanoparticlesMagnetite NanoparticlesAnimalsCell Culture TechniquesCell LineCentrifugation, Density GradientHumansMagnetite NanoparticlesDextranEndocytosisLysosomesSPIONsSuperparamagnetic nanoparticlesβ-hexosaminidase assay

Identifiers

PMID41082114

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.