Evidence map›Paper›PMID 41082113›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Measurement of Physiological Autophagic Flux in the Human Peripheral Blood Mononuclear Cell Pool.

Leanne K Hein, Kathryn J Hattersley, Julien Bensalem, Timothy J Sargeant

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Leanne K HeinLysosomal Health in Ageing, Hopwood Centre for Neurobiology, Lifelong Health Theme, South Australian Health and Medical Research Institute, Adelaide, SA, Australia.
Kathryn J HattersleyLysosomal Health in Ageing, Hopwood Centre for Neurobiology, Lifelong Health Theme, South Australian Health and Medical Research Institute, Adelaide, SA, Australia.
Julien BensalemLysosomal Health in Ageing, Hopwood Centre for Neurobiology, Lifelong Health Theme, South Australian Health and Medical Research Institute, Adelaide, SA, Australia.
Timothy J SargeantLysosomal Health in Ageing, Hopwood Centre for Neurobiology, Lifelong Health Theme, South Australian Health and Medical Research Institute, Adelaide, SA, Australia. Tim.Sargeant@sahmri.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autophagic flux, a crucial cellular process that is often overlooked in its significance for human health, has been linked to various chronic diseases, including cancer, heart disease, and dementia. Despite its importance, direct measurement in humans has been lacking, which has hindered translational efforts. Here, we describe a protocol for the measurement of human autophagic flux in blood. By treating whole blood with chloroquine and isolating peripheral blood mononuclear cells, autophagic flux can be measured by quantifying the autophagy protein LC3BII. This approach preserves individual genetic and nutritional parameters. This method can be used to identify factors influencing autophagic flux in humans, facilitating clinical translation and potentially serving as a biomarker for age-related chronic diseases. In this protocol, we describe detailed methodology, along with risks and limitations of this technique.

Indexed as

AutophagyLeukocytes, MononuclearBiomarkersChloroquineHumansMicrotubule-Associated ProteinsBiomarkersChloroquineMAP1LC3B protein, humanMicrotubule-Associated ProteinsAutophagic fluxAutophagyBloodHumanLysosomePBMC

Identifiers

PMID41082113

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.