ArticleJournal of assisted reproduction and genetics2025
Distinct post-insemination dynamics between fresh and frozen-derived gametes using testicular sperm.
Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Fresh versus frozen-thawed testicular sperm for intracytoplasmic sperm injection in men with non-obstructive azoospermia: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate differences in post-insemination events between fresh and frozen-derived gametes when testicular sperm extraction (TESE) is used.
methodA retrospective cohort study including 980 oocytes retrieved across 72 cycles from 30 couples, between January 2017 and September 2023. Gamete sources were fresh (fresh-TESE) or frozen-thawed-TESE (frozen-TESE) from the same patients with non-obstructive azoospermia (NOA), and fresh or vitrified (frozen) oocytes from the same female partner. Following insemination by TESE-sperm, embryos were cultured in a time-lapse (TL) incubator. Resulting blastocysts underwent trophectoderm (TE) biopsy for preimplantation genetic testing for aneuploidy (PGT-A) by next-generation sequencing (NGS). Fertilization, usable blastocysts, and euploidy rates were recorded, and morphokinetic key parameters were annotated.
resultsOocyte/sperm dyads using frozen-TESE showed an association with lower fertilization (OR 0.64, 95% CI 0.45-0.89, P = 0.008) and lower blastulation/MII (OR 0.53, 95% CI 0.37-0.77, P = 0.001), but similar blastulation/2PN (OR 0.69, 95% CI 0.45-1.07, P = 0.09). After accounting for female age, euploidy per tested blastocyst was similar whether fresh or frozen-TESE was used, but frozen oocyte use showed no association with these outcomes. The use of frozen dyads was associated with longer duration of 2PN after tPNa to tPNf: in frozen-TESE, 2.10 h longer (95% CI 0.55-3.64, P = 0.008) and in vitrified oocytes, 2.34 h longer (95% CI 0.31-4.36, P = 0.024). Slight morphokinetic differences between fresh and frozen-TESE were observed during subsequent developmental stages.
conclusionsSynchronizing fresh-TESE with oocyte retrieval, when possible, appears safer to maximize fertilization rates. Regardless of the gamete type, frozen-sourced sperm or oocyte impact 2PN-lag time, possibly indicating nuclear repair mechanisms post-freezing, requiring further investigation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.