Evidence map›Paper›PMID 41081960›Full record

ArticleFunctional & integrative genomics2025

Multi-Omics analysis unveils the regulatory mechanism of ZNF423 in bovine intramuscular fat formation.

Xiaoyu Song, Jiupan Zhang, Yaping Song, Sayed Haidar Abbas Raza, Sameer Dinkar Pant, Linsen Zan, Guijie Zhang, Dawei Wei

Abstract read
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In one paragraph

Article in Functional & integrative genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoyu SongCollege of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China.
Jiupan ZhangInstitute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750021, China.
Yaping SongCollege of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China.
Sayed Haidar Abbas RazaState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510006, PR China.
Sameer Dinkar PantGulbali Institute, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia.
Linsen ZanCollege of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling, 712100, China.
Guijie ZhangCollege of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China. guijiezhang@nxu.edu.cn.
Dawei WeiCollege of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China. weidawei@nxu.edu.cn.

Funding

the Natural Science Foundation of China 32202641, 32460819, 31960672
6 · The paper itself

Abstract

Marbling (intramuscular fat) is a crucial determinant of beef palatability. Zinc finger protein 423 (ZNF423) plays a key role in adipocyte differentiation and lipid metabolism, but its regulatory mechanisms, particularly in bovine adipocytes, remain poorly understood. This study demonstrates that si-ZNF423 inhibits adipocyte proliferation by disrupting the G1/S transition and down-regulating key proliferation markers. Additionally, ZNF423 knockdown suppresses the expression of major adipogenic transcription factors (PPARγ, C/EBPβ) and lipid metabolism genes (SCD1, FABP4), resulting in reduced triglyceride (TG) accumulation and impaired lipid droplet formation. Transcriptomic and non-targeted metabolomic analyses indicated that ZNF423 regulates adipogenesis through pathways involving retinoic acid (RA) signaling. ChIP and dual luciferase assays confirmed that ZNF423 directly binds to the promoters of SLC45A2 and SNCG, identifying these genes as novel downstream targets. Notably, RA treatment compensates for the effects of si-ZNF423 knockdown, not only restoring and significantly enhancing ZNF423 activity and downstream adipogenic processes, highlighting a conserved interplay between ZNF423 and RA signaling in bovine adipogenesis. This study provides new insights into the molecular mechanisms of bovine fat development, offering potential targets for improving beef quality.

Indexed as

AdipogenesisAdipocytesAnimalsCattleCell ProliferationLipid MetabolismMultiomicsSignal TransductionTretinoinTretinoinAdipocytesBovineRAZNF423

Identifiers

PMID41081960

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.