ArticleClinical oral investigations2025
Antimicrobial, toxicity, and cellular interactions of bioceramic sealers in periodontal ligament: in vitro insights.
Article in Clinical oral investigations, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The trial behind it
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Who cites it
3 citing papers in PubMed.
- Effect of dentin pre-treatment with Bioglass F18 suspensions on surface characteristics and bond strength of endodontic sealers.Clinical oral investigations · 2026Article
- Cytotoxicity of Root Canal Sealers and Potential Clinical Implications: A Comprehensive Systematic Review of In Vitro Studies.Journal of clinical medicine · 2026Review
- Periapical Lesion Healing After Retreatment and Root Canal Filling with a Bioceramic-Based Sealer: A Randomized Clinical Study with 1-Year Follow-Up.Journal of clinical medicine · 2025Article
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThis study aimed to evaluate the antimicrobial, cytotoxic, proliferative, migratory, and immunomodulatory potential of Bio-C Sealer and Bio-C Sealer ion + extracts compared to AH Plus, using human periodontal ligament stem cells (hPDLSCs). MATERIALS AND
methodsInitially, the minimum concentrations of sealer extracts required to inhibit the growth of Enterococcus faecalis and Candida albicans were determined. hPDLSCs were then obtained from extracted third molars of young donors and cultured under standard conditions. Cells were characterized by flow cytometry using mesenchymal stem cell markers (CD44, CD73, CD90, CD105) and a negative cocktail of hematopoietic markers, following the minimal criteria of the International Society for Cellular Therapy. Subsequently, the cytotoxic, proliferative, and migratory effects on hPDLSCs were assessed using MTT, Trypan Blue exclusion and scratch assays. Finally, the gene expression profile of hPDLSCs exposed to sealer extracts was analyzed by qPCR. Statistical differences were evaluated using one-way and two-way ANOVA followed by Tukey's post hoc tests.
resultsAH Plus and Bio-C Sealer ion + inhibited 100% of E. faecalis growth at extract dilutions of 1:1, 1:2, and 1:4. However, only undiluted extracts of AH Plus, Bio-C Sealer, and Bio-C Sealer ion + completely inhibited C. albicans growth. The cytotoxicity of all sealers was dose-dependent in hPDLSC cultures, with lower cell viability observed in undiluted extracts: AH Plus (55%), Bio-C Sealer (69%), and Bio-C Sealer ion + (72%) (p < 0.0001). Bio-C Sealer ion + extract (1:4) promoted the highest rate of cell migration and proliferation after 48 h (p < 0.0001). Regarding gene expression, bioceramic sealers-maintained levels of TNF-α, IL-6, and IL-10 similar to the control condition, whereas AH Plus upregulated these cytokines.
conclusionsBioceramic sealers exhibited satisfactory antimicrobial activity with moderate cytotoxicity. Moreover, they enhanced wound closure and maintained cytokine expression at levels comparable to the control condition in periodontal ligament stem cells. CLINICAL RELEVANCE: Bioceramic sealers such as Bio-C Sealer and Bio-C Sealer ion + demonstrated superior biological compatibility compared with AH Plus, including lower cytotoxicity, enhanced cell migration and proliferation, and effective control of inflammatory cytokine induction. These properties suggest greater safety for endodontic use and potential to promote tissue healing and long-term clinical success.
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Registered trials
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