Evidence map›Paper›PMID 41080444›Full record

ArticleBio-protocol2025

Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid-Liquid Phase Separation.

Kaixin Wang, Guangjie Zhang, Lei Zhang, Yugang Bai, Tong Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kaixin WangState Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, China.
Guangjie ZhangState Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, China.
Lei ZhangState Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, China.
Yugang BaiState Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, China.
Tong WuState Key Laboratory of Chemo- and Bio-Sensing, School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Artificial metalloenzymes (ArMs) hold great promise for expanding the toolbox of non-natural transformations usable in living systems, such as cells, plants, and animals. However, their practical application remains challenging, primarily due to their unsatisfactory stability and inefficient intracellular assembly. We recently reported a new strategy, called artificial metalloenzymes in artificial sanctuaries (ArMAS) through liquid-liquid phase separation (LLPS), to enhance the performance of ArMs in cells by placing them in more friendly artificial microenvironments. Here, this protocol describes the detailed method for using this ArMAS-LLPS strategy, a robust way to create artificial compartments using an ArM protein scaffold through LLPS and construct ArMs within using self-labeling cofactor anchoring reactions. In detail, in

Indexed as

Artificial metalloenzymeCompartmentalizationLiquid–liquid phase separationMicroenvironmentWhole-cell catalyst

Identifiers

PMID41080444
PMCPMC12514139

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.