Complex Investigation of the Similarities and Differences between Ten Commercially Available Human Serum Albumin Preparations.

Rita Jakabfi-Csepregi, Zoltán Nagymihály, Zoltán Horváth-Szalai, Balázs Szirmay, Éva Varga-Visi, Omeralfaroug Ali, Edward Agyarko, András Szabó, Miklós Poór

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Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Rita Jakabfi-CsepregiDepartment of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.

Zoltán NagymihályMolecular Medicine Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs H-7624, Hungary.

Zoltán Horváth-SzalaiDepartment of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.

Balázs SzirmayDepartment of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.

Éva Varga-VisiAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Hungarian University of Agriculture and Life Sciences, Guba Sándor u. 40, Kaposvár H-7400, Hungary.

Omeralfaroug AliAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Hungarian University of Agriculture and Life Sciences, Guba Sándor u. 40, Kaposvár H-7400, Hungary.

Edward AgyarkoAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Hungarian University of Agriculture and Life Sciences, Guba Sándor u. 40, Kaposvár H-7400, Hungary.

András SzabóAgribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Hungarian University of Agriculture and Life Sciences, Guba Sándor u. 40, Kaposvár H-7400, Hungary.ORCID https://orcid.org/0000-0002-5315-0024

Miklós PoórDepartment of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs H-7624, Hungary.ORCID https://orcid.org/0000-0003-1425-7459

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human serum albumin (HSA) maintains the oncotic pressure in the blood, also having buffer and antioxidant functions. Furthermore, numerous ligand molecules are circulating dominantly in albumin-bound form in the intravascular water space. The formation of stable ligand-albumin complexes commonly has significant physiological, pharmacological, and/or toxicological importance. Regarding ligand-HSA interactions, certain studies show controversial results that may have partly resulted from the differences between the protein preparations applied. To test this hypothesis, 10 HSA preparations were obtained, and then their albumin and free fatty acid content, fructosamine and free thiol levels, antioxidant capacity, fluorescence emission spectra, and ligand binding ability were examined. Our results demonstrate that sometimes even major differences can be observed when we compare these proteins. Therefore, the precise specification and the careful selection of HSA preparations seem to be reasonable.

Identifiers

PMID41078838

DOI10.1021/acsomega.5c06541

PMCPMC12509120

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