Evidence map›Paper›PMID 41078598›Full record

ArticleInternational journal of particle therapy2025

Bystander Effects in Osteoblasts and Osteoclasts: Comparison Between X- and Proton-Irradiation.

Noriyuki Okonogi, Yukako S Otani, Keisuke Otani, Paola Divieti Pajevic, Yunhe Xie, Nicolas Depauw, Harald Paganetti, Jan Schuemann, David T Miyamoto, Kathryn D Held and 1 more

Abstract read
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In one paragraph

Article in International journal of particle therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Noriyuki OkonogiDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Yukako S OtaniDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Keisuke OtaniDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Paola Divieti PajevicDepartment of Translational Dental Medicine, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA.
Yunhe XieDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Nicolas DepauwDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Harald PaganettiDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Jan SchuemannDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
David T MiyamotoDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Kathryn D HeldDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.
Aimee L McNamaraDepartment of Radiation Oncology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: This study investigated radiation-induced bystander responses (RIBR) in osteoblasts and osteoclasts and compared x-rays with protons regarding RIBR effects. Materials and Methods: MC3T3-E1 subclone 4 (preosteoblasts), differentiated MC3T3-E1 (osteoblasts), and differentiated RAW264.7 (osteoclasts) were irradiated with x-rays (225 kVp) and protons with a linear energy transfer of -2.3 keV/µm. A trans-well insert coculture system, allowing the transfer of soluble factors without contact between irradiated and nonirradiated cells, was used to study medium-mediated RIBR. The range of intended dose was 0.5 to 2 Gy for both x-ray and proton-irradiation. The RIBRs of nonirradiated cells were evaluated based on the expression of 53BP1 at 1 h postirradiation and micronuclei assay at 72 h or later. Results: RIBR was evident in bystander cells regardless of the irradiated/nonirradiated cell combination. The percentage of 53BP1-positive cells was 3.2% to 4.2% in controls and 8.6% to 11.7% in bystander cells ( Conclusion: This study provides preliminary evidence that RIBR occurs in osteoblasts and osteoclasts, irrespective of cell combination, dose, or radiation type, suggesting that both x-rays and protons may induce comparable bystander effects in bone-related cells.

Indexed as

Bone cellsIrradiationProton beam irradiationRadiation-induced bystander responsesX-ray

Identifiers

PMID41078598
PMCPMC12508861

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.