Evidence map›Paper›PMID 41077197›Full record

ArticleThe Journal of infection2025

Age-related severity of nontuberculous mycobacterial lung disease is mediated by aberrant macrophage responses and lung microbial dysbiosis.

Ethan G Napier, Brianna M Doratt, Isaac R Cinco, Ethan V Stuart, Rebecca D Geron, Michael H Davies, Delphine C Malherbe, Steven G Kohama, Luiz Bermudez, Kevin L Winthrop and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infection, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ethan G NapierDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Brianna M DorattDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Isaac R CincoDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Ethan V StuartDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Rebecca D GeronDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Michael H DaviesDivision of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.
Delphine C MalherbeDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA.
Steven G KohamaDivision of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.
Luiz BermudezDepartment of Microbiology, College of Sciences, Oregon State University, Corvallis, OR, USA.
Kevin L WinthropDivision of Infectious Diseases, Schools of Medicine and Public Health, Oregon Health and Sciences University, Portland, OR, USA.
Cristina FussDepartment of Radiology and Biomedical Imaging, School of Medicine, Yale, New Haven, CT, USA.
Eliot R SpindelDivision of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.
Ilhem MessaoudiDepartment of Microbiology, Immunology, and Molecular Genetics, College of Medicine, University of Kentucky, Lexington, KY, USA. Electronic address: ilhem.messaoudi@uky.edu.

Funding

Upgrade of confocal microscopy at the Oregon National Primate Research CenterP51OD011092 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Bonnie J. Nagel · 2012 to 2026
$203.9M
University of Kentucky Markey Cancer Center Support Grant ECIA SupplementP30CA177558 · NCI · UNIVERSITY OF KENTUCKY · PI KATHLEEN L. O'CONNOR · 2013 to 2026
$38.3M
Mechanisms of increased susceptibility to pulmonary Nontuberculous Mycobacterial disease in the elderlyR01AI152258 · NIAID · UNIVERSITY OF KENTUCKY · PI MESSAOUDI, ILHEM · 2020 to 2024
$3.5M
Interdisciplinary Training in Alcohol ResearchT32AA027488 · NIAAA · UNIVERSITY OF KENTUCKY · PI Mark T Fillmore, Mark A Prendergast · 2019 to 2026
$2.0M
NCI NIH HHS P30 CA177558NIAAA NIH HHS T32 AA027488NIAID NIH HHS R01 AI152258NIH HHS P51 OD011092
6 · The paper itself

Abstract

objectivesAlthough cases of nontuberculous mycobacteria (NTM) pulmonary disease (NTMPD) are rapidly increasing primarily in individuals over the age of 65, the host factors leading to higher occurrence in immunocompetent older patients remain elusive. This study aims to elucidate host factors leading to higher nontuberculous mycobacterial pulmonary disease occurrence in older immunocompetent patients.

methodsTo address these challenges, we used a rhesus macaque model developed by our laboratory where macaques were infected via intrabronchial inoculation with M. avium subsp. hominissuis (MAH). Disease progression, bacterial load, microbial community compositions, and host responses were monitored longitudinally using computed tomography, culturing, 16S amplicon sequencing, histology, flow cytometry, and single cell RNA sequencing.

resultsDespite comparable kinetics of bacterial clearance, computed tomography scans indicated more severe radiological outcomes in older animals which mounted predominantly an inflammatory acute phase response rather than a Th1 response. Single cell RNA sequencing indicated a persistent inflammatory signature in aged animals. Microbial community analysis revealed an age-mediated loss of an uncultured Tropheryma species that was inversely correlated with severity of radiographic changes.

conclusionsThese data reveal that nontuberculous mycobacterial pulmonary disease severity in the aged animals is driven by dysregulated inflammatory responses and dysbiosis of the lung microbiome.

Indexed as

DysbiosisLungLung DiseasesMacrophagesMycobacterium Infections, NontuberculousAge FactorsAnimalsBacterial LoadDisease Models, AnimalFemaleMacaca mulattaMaleMicrobiotaNontuberculous MycobacteriaRNA, Ribosomal, 16SSeverity of Illness IndexRNA, Ribosomal, 16SAgingMicrobiomeNontuberculous mycobacteriaRhesus macaqueSingle cell RNA sequencing

Identifiers

PMID41077197
PMCPMC12666862

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.