Evidence map›Paper›PMID 41076118›Full record

ArticleJournal of advanced research2026

Histone demethylase CsJMJ12 enhances resistance of Navel orange to Penicillium digitatum via H3K27me3 demethylation.

Jing Zeng, Ting Li, Mengting Liu, Jiechun Peng, Xiaoyan Duan, Zhiwei Li, Guoxiang Jiang, Jianbo Xiao, Yueming Jiang, Xuewu Duan

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In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jing ZengState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zengjing@scbg.ac.cn.
Ting LiState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: liting0621@scbg.ac.cn.
Mengting LiuState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: liumengting@scbg.ac.cn.
Jiechun PengState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: pengjiechun@scbg.ac.cn.
Xiaoyan DuanState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: duanxiaoyan@scbg.ac.cn.
Zhiwei LiState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: lizhiwei@scbg.ac.cn.
Guoxiang JiangState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: gxjiang@scbg.ac.cn.
Jianbo XiaoUniversidade de Vigo, Department of Analytical and Food Chemistry, Faculty of Sciences, Ourense 32004, Spain. Electronic address: jianboxiao@uvigo.gal.
Yueming JiangState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: ymjiang@scbg.ac.cn.
Xuewu DuanState Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China; Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. Electronic address: xwduan@scbg.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionHistone methylation is essential for plant growth and development, and adaptation to biotic and abiotic stresses. However, its role in postharvest disease resistance in fruits remains poorly understood. The study aimed to investigate the involvement of histone demethylase CsJMJ12 in enhancing postharvest resistance to Penicillium digitatum in Navel orange (Citrus sinensis).

methodsJumonji (JmjC) domain-containing proteins (JMJs) potentially involved in resistance to P. digitatum in Navel orange were identified using bioinformatics analysis, artificial inoculation and RT-qPCR. The role of JMJ12 in disease resistance and its underlying mechanism were further investigated through a series of assays, including subcellular location, Western blotting, immunofluorescence staining, transient overexpression, RNA-seq, widely targeted metabolomics, chromatin Immunoprecipitation (ChIP)-qPCR.

resultsA total of 17 JMJs were identified from the citrus genome. In Navel orange infected with P. digitatum, the expression of CsJMJ12 was significantly upregulated. CsJMJ12 is localized to the nuclei and functions as a site-specific H3K27me3 histone demethylase. Overexpressing CsJMJ12 transiently in fruit resulted in increased resistance to P. digitatum, accompanied by reduced global H3K27me3 levels. Transcriptome sequencing revealed that CsJMJ12 regulates multiple defense pathways, including phenylpropanoid biosynthesis, lignin biosynthesis, cell wall organization, pathogenesis-related protein, and hormone signaling. Metabolomics showed that CsJMJ12 regulates the accumulation of disease-resistant metabolites such as dihydrocoumarin, caffeoylputrescine, prenylnaringenin, quinic acid and syringic acid in phenylpropanoid biosynthesis pathways. Furthermore, CsJMJ12 promoted the expression of key defense-associated genes, including POD, COMT1, LAC7L, SAME, SAMT, CHI, GT2 and CSL5, by demethylating H3K27me3 at their chromatin loci, thereby enhancing fruit resistance to P. digitatum.

conclusionsThis study identifies CsJMJ12 as a potential epigenetic regulator of P. digitatum resistance in Navel orange. Our findings provide valuable insights into the molecular mechanism of pathogen defense in sweet orange (Citrus sinensis). Further studies are needed to explore its broader application in agricultural practices and postharvest disease management.

Indexed as

Citrus sinensisDisease ResistanceHistone DemethylasesHistonesJumonji Domain-Containing Histone DemethylasesPenicilliumPlant DiseasesPlant ProteinsDemethylationFruitGene Expression Regulation, PlantHistone DemethylasesHistonesJumonji Domain-Containing Histone DemethylasesPlant ProteinsH3K27me3JmjC domain-containing proteinsNavel orangePenicillium digitatum

Identifiers

PMID41076118
PMCPMC13316354

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.