ArticleBiotechnology progress
Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme-derived peptide tag.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Enhanced deoxyviolacein bioproduction via enzyme condensation with a yeast glycolytic enzyme-derived peptide tag.Biotechnology progressArticle
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5 authors.
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Abstract
In microbial production, regulating endogenous and exogenous synthetic pathways is essential. Techniques to induce intracellular enzyme condensation have attracted attention as a means to increase apparent enzyme activity with minimal transcriptional and translational burden on the cell. Artificial enzyme condensation can be induced by tagging enzymes with specific proteins or peptides. In our previous study, we identified novel peptide tags derived from condensate-forming Saccharomyces cerevisiae glycolytic enzymes and their potential use in controlling intracellular metabolism by inducing artificial condensates in S. cerevisiae cells. Herein, we evaluated the condensate formation of two enzymes using peptide tags and the branched violacein biosynthetic pathway from Pseudoalteromonas luteoviolacea in S. cerevisiae, and tested the effects of modulating biosynthesis to increase the production of deoxyviolacein, a byproduct with antibacterial and anticancer activities. We used several protein and peptide tags with a simplified expression system, and all the tags successfully induced artificial condensate formation in the cell. Additionally, introducing a short peptide tag successfully increased deoxyviolacein production by approximately twofold, displaying a higher efficacy compared to FUS
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