ArticleMolecular biology reports2025
Effect of fish biomass on environmental DNA shedding and degradation.
Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Research on Quantitative Analysis Technology for Pacific White Shrimp Density Based on Environmental DNA.Ecology and evolution · 2026Article
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Authors and funding
3 authors.
Funding
Abstract
backgroundEnvironmental DNA in freshwater samples is a promising tool for assessing the relationship between biomass and eDNA concentration. We performed a quantitative PCR in experimental tanks to verify the influence of density/biomass on the release and decay of DNA in water samples. METHODS AND
resultsThe three densities used were one fish (0.416 ± 0.075 g L⁻¹), two fish (0.778 ± 0.041 g L⁻¹), and three fish (1.277 ± 0.165 g L⁻¹) in triplicate. We collected water samples at 0, 24, 48, 72, and 96 h (with and without fish) for analysis. Temperature, pH, and oxygen remained constant, with only total ammonia increasing with fish density. We verified that eDNA concentrations increased with density/biomass, revealing a positive correlation in the fish assays. After 24 h, eDNA stabilized, reaching equilibrium. We observed a significant drop in the eDNA concentration within 24 h of removing the fish. The persistence of eDNA increased from high to low densities, indicating a strong correlation with the decay rate constant.
conclusionsHerein, the results demonstrate the potential use of eDNA samples for monitoring and managing fish in both aquaculture and wild environments. However, further studies on different types of water (black, clear, and white waters) in the Amazon are necessary to facilitate the routine application of eDNA in the region.
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