Evidence map›Paper›PMID 41075003›Full record

ArticleMikrochimica acta2025

Comparative aptamer screening for patulin and the development of sensitive colorimetric assay for fruit juice analysis.

Haijun Pan, Ruijing Liu, Dongjie Liu, Lukai Ma, Qin Wang, Gengsheng Xiao, Feng Wang

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Haijun PanCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China.
Ruijing LiuGuangdong Engineering Technology Research Center for Pre-Cooked Food Processing and Quality Evaluation, Shunde Polytechnic University, Foshan, 528333, China.
Dongjie LiuCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China.
Lukai MaCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China. m1991lk@163.com.
Qin WangCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China.
Gengsheng XiaoCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China.
Feng WangCollege of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China. wangfeng_sp@163.com.

Funding

Natural Science Foundation of China 32202175Open Fund of Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs 2025nong003-1the Guangdong Province Key Construction Discipline Research Ability Improvement Project 2022ZDJS021, 2024ZDJS006the Guangdong Province Modern Agricultural Industry Common and Critical Technologies R&D Innovation Team Development Project (Processing and Preservation Common and Critical Technologies) 2024CXTD16
6 · The paper itself

Abstract

A gold nanoparticle-based colorimetric assay was developed for patulin, a mycotoxin, detection in fruit juice. Eight patulin-specific aptamers were rigorously screened using molecular docking and specific binding analyses to select the optimal candidate. The optimized assay exhibited a wide linear detection range of 5.0-200.0 ng/mL in the buffer reaction system and high specificity for patulin. Its reliability for real-world samples was validated by achieving good recoveries (83.6%-90.7%) in spiked apple and hawthorn juice, consistent with HPLC results. This study highlights an effective methodology for screening high-performance aptamers for food contaminants and a novel strategy for high-throughput food safety assessment targeting patulin.

Indexed as

Aptamers, NucleotideColorimetryFood ContaminationFruit and Vegetable JuicesPatulinGoldLimit of DetectionMetal NanoparticlesMolecular Docking SimulationAptamers, NucleotideGoldPatulinAptamerColorimetric assayGold nanoparticlesMolecular dockingPatulin

Identifiers

PMID41075003

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.