ArticleEuropean journal of medical research2025
Saliva as a potential and non-invasive approach to identify upregulated genes associated with comorbidities of T1DM: a brief report.
Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Identification of novel candidate neural genes for diet-induced obesity in outbred heterogeneous stock rats.Research square · 2026Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
backgroundOne of the main challenges in Type 1 diabetes mellitus (T1DM) research is the sample size of the participants and the invasive process of collecting an adequate number of blood samples from young patients with T1DM. Therefore, it is of great interest to investigate the possibility of using saliva as a non-invasive tool to investigate the genetic factors that are associated with T1DM comorbidities. The present study aims to identify differentially expressed genes (DEGs) in saliva samples of T1DM patients with various comorbidities using transcriptomic profiling.
methodsA total of 56 participants were recruited from the University Hospital Sharjah, Dubai Hospital and Rashid Hospital, United Arab Emirates. Participants were divided into various groups: Control Group: healthy Emirati (n = 13), Group 1: patients with T1DM without any comorbidities (G1; n = 14), Group 2: patients with T1DM and hyperlipidemia (G2; n = 10), Group 3: patients with T1DM and neuropathy (G3; n = 5), Group 4: patients with T1DM and ketoacidosis (G4; n = 6), Group 5: patients with T1DM and hypothyroidism (G5; n = 6) and Group 6: patients with T1DM and polycystic ovary syndrome (PCOS, G6; n = 5). Saliva samples and blood were collected from all participants and RNA was extracted for transcriptomic analysis.
resultsThe transcriptomic analysis of saliva samples of T1DM patients showed several DEGs that were associated with T1DM comorbidities. Interestingly, the number of upregulated genes in blood (n = 350) and saliva (n = 353) was comparable in the PCOS group (G6). In addition, the number of DEGs was more than double in saliva (n = 270) compared to blood (n = 118) samples in the ketoacidosis group (G4). Ten common upregulated genes between saliva and blood were identified in T1DM patients with hyperlipidemia (G2): LYPD5, TCF15, PRKY, CYHR1, CCDC173, CHRNA5, MIAT, HBA2, RAD54L, and TSHZ3. In addition, two common upregulated genes were identified in the neuropathy group (G3): KCTD19, FAM209B. Moreover, the following upregulated genes were observed in ketoacidosis (G4), hypothyroidism (G5) and PCOS (G6): (NCKAP5, ADAD2, C20orf144), (ZFY, KCTD19, P2RY14, RPS60) and (C4orf19, RSAD2, MEGF10, CMPK2, CHRNA5, TSHZ3, CCDC77, CLEC12A, RTP4), respectively.
conclusionsThe present findings demonstrated that the number of upregulated genes can be comparable between saliva and blood of T1DM patients with comorbidities, and in some groups, the number of upregulated genes was higher in the saliva compared to blood, emphasizing that saliva can be a potential non-invasive tool to identify DEGs in T1DM comorbidities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.