ArticleMethods in molecular biology (Clifton, N.J.)2026
Quantification of Tissue and Circulating MicroRNAs by Droplet Digital PCR.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Circulating miRNAs for glioblastoma monitoring: from biofluid to clinical decision.Molecular therapy. Oncology · 2026Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are gene expression regulators that play a fundamental role in developmental and biological processes. Dysregulated expression of miRNAs has been associated with most human conditions, and their accurate quantification is an essential activity in molecular biology laboratories. Indeed, the levels of specific tissue or circulating miRNAs have been tested as disease biomarkers in many clinical and experimental settings. Droplet digital PCR (ddPCR) technology is a sensitive and accurate method to obtain the absolute or relative quantification of specific miRNAs, bypassing several issues related to low abundance targets and PCR efficiency. This chapter addresses the workflow and methods for miRNA quantification in formalin-fixed paraffin-embedded (FFPE) samples, cells, and plasma/serum/extracellular vesicles using EvaGreen-based ddPCR or probe-based ddPCR, as well as how to analyze and interpret results. In addition, we provide a ddPCR method to quantify miRNA isoforms (isomiRs).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.