ArticleMethods in molecular biology (Clifton, N.J.)2026
Crystal Digital PCR™-Based Single Nucleus Pollen Genotyping in Barley (Hordeum vulgare L.) to Measure Meiotic Crossover Rates in Defined Chromosomal Intervals.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genotyping-based meiotic crossover (CO) rate measurement within defined chromosomal regions typically relies on the segregation analysis of linked markers (polymorphism) in hybrid offspring populations. Pollen (male gametes) nucleus genotyping can be an alternative as pollen is abundant even from single individuals. Hence, CO rates can be measured without segregation distortion and growing offspring populations. Here, we present a protocol for meiotic recombination and CO interference rate measurements within two-linked chromosomal regions in hybrid barley pollen nuclei using multiplex Crystal digital PCR (dPCR)-based single pollen nucleus genotyping on the Naica™ Crystal Digital PCR platform. It consists of hybrid pollen isolation, flow sorting of single pollen nuclei, and their genotyping through dPCR.
Indexed as
Identifiers
41073860What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.