Evidence map›Paper›PMID 41073553›Full record

ArticleScientific reports2025

Nanobody conjugated with dendrimer nanoparticles effectively neutralizes tick-borne encephalitis virus.

Jana Jozefiaková, Amod Kulkarni, Dinesh Dhumal, Ádám Kevély, Eva Nováková, Kamila Koči, Ling Peng, Juraj Koči, Mangesh Bhide

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jana JozefiakováLaboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04181, Slovakia.
Amod KulkarniLaboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04181, Slovakia.
Dinesh DhumalCentre Interdisciplinaire de Nanoscience de Marseille, Aix-Marseille University, CNRS, UMR 7325 CINaM, Marseille, 13288, France.
Ádám KevélyInstitute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, 84510, Slovakia.
Eva NovákováInstitute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, 84510, Slovakia.
Kamila KočiDepartment of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, Bratislava, 84215, Slovakia.
Ling PengCentre Interdisciplinaire de Nanoscience de Marseille, Aix-Marseille University, CNRS, UMR 7325 CINaM, Marseille, 13288, France.
Juraj KočiInstitute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, 84510, Slovakia.
Mangesh BhideLaboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice, 04181, Slovakia. bhidemangesh@gmail.com.

Funding

Agentúra na Podporu Výskumu a Vývoja APVV-22-0084Agentúra na Podporu Výskumu a Vývoja APVV-23-0348EuroNanoMed III EURONANOMED2021-105International Center for Genetic Engineering and Biotechnology ICGEB CRP/SVK24-02Vedecká Grantová Agentúra MŠVVaŠ SR a SAV VEGA1/0381/23
6 · The paper itself

Abstract

Tick-borne encephalitis virus (TBEV) is a neurotropic flavivirus causing severe meningitis, encephalitis, or meningoencephalitis. The blood-brain barrier (BBB) prevents therapeutic agents from reaching the brain; therefore, specific antiviral therapy that can cross the BBB and treat TBEV-induced infection is urgently needed. In this study, nanobodies (NBs) targeting domain III (DIII) of the envelope (E) glycoprotein of TBEV were developed and conjugated to drug delivery nanosystems based on self-assembling dendrimer nanomicelles (DNs) and gold nanoparticles (AuNs) to facilitate BBB passage and viral neutralization. NBs were derived from the variable region of heavy chain-only llama antibodies and panned against DIII using phage display. The most active NBs were conjugated with DNs and AuNs. Remarkably, the dendrimer nanosystem decorated with the nanobody clone TA3 (TA3-DNs) and the peptide Angiopep-2 effectively crossed the BBB model and neutralized TBEV. Therefore, TA3-DNs constitute a promising candidate for treating TBEV, and merit further validation in animal models for future translational studies.

Indexed as

Antibodies, NeutralizingDendrimersEncephalitis, Tick-BorneEncephalitis Viruses, Tick-BorneMetal NanoparticlesSingle-Domain AntibodiesAnimalsAntibodies, ViralAntiviral AgentsBlood-Brain BarrierGoldHumansMiceNanoparticlesViral Envelope ProteinsAntibodies, NeutralizingAntibodies, ViralAntiviral AgentsDendrimersGoldSingle-Domain AntibodiesViral Envelope ProteinsBlood-brain barrierDendrimer nanosystemGold nanoparticlesNanobodiesTick-borne encephalitis virus

Identifiers

PMID41073553
PMCPMC12514040

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.